On ras gene function in yeast.
On ras gene function in yeast.
复制标题
酵母中ras基因的功能。
DOI：
10.1073/pnas.82.14.4740
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发表时间：
1985
影响因子：
11.1
通讯作者：
Fraenkel,DG
中科院分区：
文献类型：
--
作者：
Fraenkel,DG
酿酒酵母含有两个RAS基因，即RAS1和RAS2。RAS2的插入突变（RAS2::LEU2）不影响葡萄糖基培养基上的生长，但它确实阻止了丙酮酸或其他非碳水化合物碳源培养基上的生长。这种缺陷对pH值敏感，在pH值7及以上时最为严重。ras2::LEU2突变也导致糖原水平在葡萄糖耗竭后生长的低抑制阶段显著升高。丙酮酸恢复生长的选择产生非连锁抑制突变。一些抑制因子还能将糖原和海藻糖（酵母中的另一种储备碳水化合物）降低到比野生型菌株低得多的水平。这些抑制突变不抑制ras1 - ras2双突变体的致死率。结果间接符合酵母RAS2调控腺苷酸环化酶G蛋白活性的理论。
Saccharomyces cerevisiae contains two RAS genes, RAS1 and RAS2. An insertion mutation in RAS2 (ras2::LEU2) does not affect growth on glucose based media but it does prevent growth on media with pyruvate or other noncarbohydrate carbon sources. This defect is pH sensitive and is most severe at pH 7 and above. The ras2::LEU2 mutation also causes markedly higher levels of glycogen in the derepressed phase of growth after glucose exhaustion. Selection for restoration of growth on pyruvate yields unlinked suppressor mutations. Some of the suppressors also reduce glycogen as well as trehalose (the other reserve carbohydrate in yeast) to levels much lower than those of wild-type strains. These suppressor mutations do not suppress the lethality of ras1 ras2 double mutants. The results indirectly accord with yeast RAS2 governing a G protein activity of adenylate cyclase.