Thiamine seed treatment enhances LOX expression, promotes growth and induces downy mildew disease resistance in pearl millet
Thiamine seed treatment enhances LOX expression, promotes growth and induces downy mildew disease resistance in pearl millet
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DOI：
10.1007/s10535-011-0118-3
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发表时间：
2011-07
影响因子：
1.5
通讯作者：
H. Pushpalatha;J. Sudisha;N. Geetha;K. N. Amruthesh;H. Shekar Shetty
中科院分区：
文献类型：
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作者：
H. Pushpalatha;J. Sudisha;N. Geetha;K. N. Amruthesh;H. Shekar Shetty
珍珠粟[Pennisetum glaucum（L.）[R.Br.]感病品种7042S用5、10、15、20和25 mM浓度的硫胺素处理，并测试生长促进和霜霉病抗性。用20 mM硫胺素处理种子，在温室和田间条件下分别获得72%和70%的病害保护，并增强了营养和生殖生长参数。对不同时间段的珍珠粟幼苗脂氧合酶（LOX）活性分析表明，LOX活性在接种后3h（hai）开始升高，24hai时达到最大。北方分析表明，LOXmRNA在抗病幼苗中的转录积累量较高。IP18292）比感病幼苗的抗性更强。硫胺素种子处理诱导LOX基因快速表达并导致对霜霉病的显著疾病保护。
Seeds of pearl millet [Pennisetum glaucum(L). R.Br.] susceptible cv. 7042S were treated with thiamine at 5, 10, 15, 20 and 25 mM concentrations and growth promotion and downy mildew resistance were tested. Seed treatment with 20 mM thiamine resulted in 72 and 70 % disease protection under greenhouse and field conditions, respectively, and enhanced vegetative and reproductive growth parameters. Analysis of lipoxygenase (LOX) activity in inoculated pearl millet seedlings at different time intervals indicated that increased LOX activity was initiated at 3 h after inoculation (hai) and maximum activity was observed at 24 hai. Northern analysis showed thatLOXmRNA transcript accumulation was higher in the resistant seedlings (cv. IP18292) than in susceptible seedlings. Thiamine seed treatment induces rapidLOXgene expression and results in significant disease protection against downy mildew disease.