Core-Shell Upconversion Nanoparticle@Metal-Organic Framework Nanoprobes for Luminescent/Magnetic Dual-Mode Targeted Imaging
Core-Shell Upconversion Nanoparticle@Metal-Organic Framework Nanoprobes for Luminescent/Magnetic Dual-Mode Targeted Imaging
复制标题
用于发光/磁性双模式靶向成像的核壳上转换纳米粒子@金属有机框架纳米探针
DOI：
10.1002/adma.201501779
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发表时间：
2015-07-15
影响因子：
29.4
通讯作者：
Tang, Zhiyong
中科院分区：
文献类型：
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作者：
Li, Yantao;Tang, Jinglong;Tang, Zhiyong
DOI：10.1002/adma. 201501779被开发为最有用的T1加权MRI试剂。此外，与超顺磁性氧化铁纳米颗粒（SPION）偶联的UCNPs原型由于其大磁矩而被广泛应用于UCL/T2-MRI造影剂。[8]然而，Gd 3+离子的毒性、核壳结构UCNP@ FexOyNPs的不稳定性和团聚等问题仍是制约其临床应用的主要因素。[9]因此，迫切需要开发新的UCL/MRI造影剂，其具有更容易制备和功能化、更低的生物毒性、更好的生物相容性和生物降解性。[10]受此启发，我们采用不同的策略，即将单一NaYF 4：Yb，Er UCNP核结合到Fe-MIL-101_NH2 NMOF [11]壳中，以构建用作UCL/MRI示踪剂的纳米复合材料。采用两步法合成了八面体核壳结构的UCNP@ Fe MIL 101_NH2纳米结构。我们发现这种核-壳纳米复合材料可以同时呈现UCNP核的近红外光学性质和MOF壳的T2-MRI性质。核-壳纳米颗粒的表面氨基被聚乙二醇-2-氨基乙基醚乙酸（NH 2-PEGCOOH）和叶酸（FA）方便地修饰，产生显示FA部分的PEG化核-壳UCNP@ Fe-MIL-101_NH 2@ PEG-FA纳米结构（简称UMP-FA）。针对KB细胞（过表达叶酸受体（Fr））和KB荷瘤小鼠，测试UMP-FA纳米复合物的UCL/T2-MRI双模成像。
DOI: 10.1002/adma. 201501779 developed as the most useful T 1-weighted MRI agents. Also, prototypes of UCNPs coupled with superparamagnetic iron oxide NPs (SPION) have been widely applied in UCL/T 2-MRI contrast agents because of their large magnetic moment.[8] However, there are still many challenges needed to be addressed for these systems, such as the toxicity of Gd 3+ ions, and the destabilization and agglomeration of the core–shell UCNP@ Fe xO y NPs, which may precludes their clinical applications.[9] Consequently, there is an emergent requirement to develop novel UCL/MRI contrast agents with easier preparation and functionalization, less biotoxicity, better biocompatibility, and biodegradability.It is worth mentioning that recent work has successfully integrated UCNPs and MOFs into the well-defined structure via encapsulation of UCNPs within MOFs.[10] Inspired by this, here we adopt a different strategy, that is, combination of single NaYF 4: Yb, Er UCNP core into Fe-MIL-101_NH 2 NMOF [11] shell, to construct a nanocomposite material to be used as a UCL/MRI tracer. The octahedral core–shell UCNP@ Fe-MIL-101_NH 2 nanostructures were synthesized using a two-step method. We have found that the core–shell nanocomposites can simultaneously present the NIR optical property of the UCNP cores and the T 2-MRI property of the MOF shells. The surface amino groups of the core–shell NPs are conveniently modified with poly (ethylene glycol)-2-amino ethyl ether acetic acid (NH 2-PEGCOOH) and folic acid (FA), resulting in PEGylated core–shell UCNP@ Fe-MIL-101_NH 2@ PEG-FA nanostructures (UMP-FA for short) that display FA moieties. UMP-FAs nanocomposites are tested for UCL/T 2-MRI dual-mode imaging targeted at KB cells (overexpressing the folate receptor (Fr)) and KB-tumorbearing mice.