Preservation of the metaproteome: variability of protein preservation in ancient dental calculus.
Preservation of the metaproteome: variability of protein preservation in ancient dental calculus.
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DOI：
10.1080/20548923.2017.1361629
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发表时间：
2017
影响因子：
3.5
通讯作者：
Speller CF
中科院分区：
文献类型：
--
作者：
Mackie M;Hendy J;Lowe AD;Sperduti A;Holst M;Collins MJ;Speller CF
牙结石的蛋白质组学分析正在成为考古人群疾病和饮食特征的有力工具。为了更好地了解牙结石蛋白质结果的变异性，我们分析了来自三个罗马时期人群的21个样本，以比较：1）提取蛋白质的数量; 2）质谱查询的数量; 3）肽谱匹配和蛋白质鉴定的数量。我们发现结石分析和总蛋白鉴定的数量之间几乎没有相关性，以及网站位置和蛋白质保存之间没有系统的趋势。我们确定了广泛的个体变异性，这可能与结石形成和/或沉积后污染的机制，除了埋藏因素。我们的研究结果表明，牙结石确实是一个稳定的，长期的水库蛋白质，如前所述，但需要进一步的系统研究，以确定与蛋白质截留和生存在牙结石的机制。
Proteomic analysis of dental calculus is emerging as a powerful tool for disease and dietary characterisation of archaeological populations. To better understand the variability in protein results from dental calculus, we analysed 21 samples from three Roman-period populations to compare: 1) the quantity of extracted protein; 2) the number of mass spectral queries; and 3) the number of peptide spectral matches and protein identifications. We found little correlation between the quantity of calculus analysed and total protein identifications, as well as no systematic trends between site location and protein preservation. We identified a wide range of individual variability, which may be associated with the mechanisms of calculus formation and/or post-depositional contamination, in addition to taphonomic factors. Our results suggest dental calculus is indeed a stable, long-term reservoir of proteins as previously reported, but further systematic studies are needed to identify mechanisms associated with protein entrapment and survival in dental calculus.