Inductively coupled plasma mass spectrometry determination of metals in honeybee venom
Inductively coupled plasma mass spectrometry determination of metals in honeybee venom
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DOI：
10.1016/j.jpba.2008.05.033
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发表时间：
2008-11-04
影响因子：
3.4
通讯作者：
Matysiak, Jan
中科院分区：
文献类型：
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作者：
Kokot, Zenon J.;Matysiak, Jan
采用电感耦合等离子体质谱（ICP-MS）技术分析了32份蜂毒样品中选定的20种金属的污染情况，并证明了这些元素含量的差异。在分析的金属微量元素（Al、Co、Cu、Zn、Mn、Mo、B、V、Sr和Ni）中，确定了大量元素（Ca、Mg、K和Na）和有毒金属（As、Ba、Pb、Cd、Sb和Cr）。结果表明，蜂毒中的金属含量远低于这些元素的可耐受上限摄入量。此外，有毒金属污染远低于美国药典和欧洲药典规定的药物允许水平。与金属的药典试验不同，本文开发了一种多元素ICP-MS方法。为了验证所获得的数据，考虑了以下步骤和参数：校准验证，回收率，准确度，精密度，检出限（LOD），定量限（LOQ），光谱和基质干扰以及ICP-MS和GFAAS（石墨炉原子吸收光谱法）对Mn的比较。所有验证步骤都证明了结果的准确性。这很可能是第一个用ICP-MS评价蜂毒中金属含量的研究。(C) 2008 Elsevier B.V.版权所有
Inductively coupled plasma mass spectrometry (ICP-MS) technique was used to analyze the contamination of selected 20 metals in 32 samples of honeybee venom and to demonstrate differences in the content of these elements. Among the analyzed metal microelements (Al, Co, Cu, Zn, Mn, Mo, B, V, Sr and Ni), macroelements (Ca, Mg, K and Na) and toxic metals (As, Ba, Pb, Cd, Sb and Cr) were identified. The presented results showed that the metal levels in honeybee venom are much lower than the tolerable upper intake levels for the elements. Also the toxic metal contamination is much lower than the permissible levels for drugs established by the United States Pharmacopeia and the European Pharmacopeia. As opposed to the pharmacopeial tests for metals, a multi-element ICP-MS method has been developed. In order to confirm data obtained, the following steps and parameters were taken into account for the validation of the method: calibration verification, recovery, accuracy, precision, detection limit (LOD), quantitation limit (LOQ), spectral and matrix interference and comparison between ICP-MS and GFAAS (graphite furnace atomic absorption spectrometry) for Mn. All steps of validation proved the accuracy of the results.This is most likely the first study in which the metal content in honeybee venom was evaluated by ICP-MS. (C) 2008 Elsevier B.V. All rights reserved.