Engineering skin to study human disease--tissue models for cancer biology and wound repair.
Engineering skin to study human disease--tissue models for cancer biology and wound repair.
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DOI：
10.1007/b137206
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发表时间：
2007
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影响因子：
--
通讯作者：
J. Garlick
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文献类型：
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作者：
J. Garlick
最近在称为皮肤等同物的三维组织工程方面的进展， 人类皮肤的形态学和表型特性，为研究人类疾病过程提供了新的途径。 本章将提供两个这样的应用程序的概述-调查的初期发展 鳞状细胞癌的研究，以及表征人类上皮细胞在创伤过程中的反应的研究 修复.使用这些新工具来研究癌症生物学，已经表明细胞间的相互作用 三维组织结构中固有结构可以通过诱导一种状态来抑制早期癌症进展 上皮内休眠这种休眠状态可以被克服，癌症的进展可以通过改变 组织响应于肿瘤促进剂或UV照射或通过改变 肿瘤细胞或其邻近上皮细胞的细胞外基质蛋白。通过调整皮肤等效值 人类皮肤模型来研究伤口上皮再生，已经表明几个关键反应，包括 细胞增殖、迁移、分化、生长因子反应性和蛋白酶表达， 模仿人类皮肤的反应。在这种情况下，这些人类皮肤的工程模型提供了强大的 研究这些组织中发生在人类身上的疾病过程的新工具。
Recent advances in the engineering of three-dimensional tissues known as skin equivalents, that have morphologic and phenotypic properties of human skin, have provided new ways to study human disease processes. This chapter will supply an overview of two such applications – investigations of the incipient development of squamous cell cancer, and studies that have characterized the response of human epithelium during wound repair. Using these novel tools to study cancer biology, it has been shown that cell-cell interactions inherent in three-dimensional tissue architecture can suppress early cancer progression by inducing a state of intraepithelial dormancy. This dormant state can be overcome and cancer progression enabled by altering tissue organization in response to tumor promoters or UV irradiation or by modifying the interaction of tumor cells with extracellular matrix proteins or their adjacent epithelia. By adapting skin equivalent models of human skin to study wound reepithelialization, it has been shown that several key responses, including cell proliferation, migration, differentiation, growth-factor responsiveness and protease expression, will mimic the response seen in human skin. In this light, these engineered models of human skin provide powerful new tools for studying disease processes in these tissues as they occur in humans.