Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis.
Revelation of mRNAs and proteins in porcine milk exosomes by transcriptomic and proteomic analysis.
复制标题
DOI：
10.1186/s12917-017-1021-8
复制
发表时间：
2017-04-13
影响因子：
2.6
通讯作者：
Zhang YL
中科院分区：
文献类型：
--
作者：
Chen T;Xi QY;Sun JJ;Ye RS;Cheng X;Sun RP;Wang SB;Shu G;Wang LN;Zhu XT;Jiang QY;Zhang YL
牛奶是一种复杂的液体，为新生儿提供营养。最近的报道表明，乳汁富含母体来源的外泌体，其通过外泌体mRNA、miRNA和蛋白质的传递参与胎儿的生理和病理状况。由于目前还没有关于外泌体mRNA和蛋白质的相关研究，因此，我们尝试利用RNA测序和蛋白质组学分析来研究外泌体mRNA和蛋白质。共鉴定出16,304个（13,895个已知mRNA和2409个新mRNA）mRNA和639个（571个已知蛋白、66个候选蛋白和2个推定蛋白）蛋白。GO和KEGG注释表明，大部分蛋白质位于细胞质中，参与多种免疫和疾病相关通路，部分mRNA与代谢、降解和信号通路密切相关。有趣的是，19类蛋白质是组织特异性的，在胎盘、肝脏、乳汁、血浆和乳腺中检测到。COG分析将所鉴定的mRNA和蛋白质分别分为6类和23类，其中18种mRNA和10种蛋白质与细胞周期调控、细胞分裂和染色体分配有关。另外，对筛选出的14条mRNA进行qPCR鉴定，并对其中10条与免疫和细胞增殖相关的蛋白进行Western blot检测。这些结果提供了对猪乳汁外泌体mRNA和蛋白质的第一次洞察，并将促进对乳汁外泌体对婴儿的生理意义的进一步研究。本文的在线版本（doi：10.1186/s12917-017-1021-8）包含补充材料，可供授权用户使用。
Milk is a complex liquid that provides nutrition to newborns. Recent reports demonstrated that milk is enriched in maternal-derived exosomes that are involved in fetal physiological and pathological conditions by transmission of exosomal mRNAs, miRNAs and proteins. Until now, there is no such research relevant to exosomal mRNAs and proteins in porcine milk, therefore, we have attempted to investigate porcine milk exosomal mRNAs and proteins using RNA-sequencing and proteomic analysis. A total of 16,304 (13,895 known and 2,409 novel mRNAs) mRNAs and 639 (571 known, 66 candidate and 2 putative proteins) proteins were identified. GO and KEGG annotation indicated that most proteins were located in the cytoplasm and participated in many immunity and disease-related pathways, and some mRNAs were closely related to metabolisms, degradation and signaling pathways. Interestingly, 19 categories of proteins were tissue-specific and detected in placenta, liver, milk, plasma and mammary. COG analysis divided the identified mRNAs and proteins into 6 and 23 categories, respectively, 18 mRNAs and 10 proteins appeared to be involved in cell cycle control, cell division and chromosome partitioning. Additionally, 14 selected mRNAs were identified by qPCR, meanwhile, 10 proteins related to immunity and cell proliferation were detected by Western blot. These results provide the first insight into porcine milk exosomal mRNA and proteins, and will facilitate further research into the physiological significance of milk exosomes for infants. The online version of this article (doi:10.1186/s12917-017-1021-8) contains supplementary material, which is available to authorized users.