Ion Exchanger Liquid Chromatography of N-Acetylaspartic Acid and Some N-Acetylaspartyl Peptides
Ion Exchanger Liquid Chromatography of N-Acetylaspartic Acid and Some N-Acetylaspartyl Peptides
复制标题
N-乙酰天冬氨酸和一些 N-乙酰天冬氨酰肽的离子交换液相色谱法
DOI：
10.1080/01483918108059980
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发表时间：
1981
影响因子：
1.3
通讯作者：
K. Lenda
中科院分区：
文献类型：
--
作者：
K. Lenda
本文报道了一种高效液相色谱法快速分离N-乙酰天冬氨酸、N-乙酰乙酰谷氨酸和N-乙酰乙酰谷氨酰天冬氨酸的方法。该方法采用薄膜状离子交换柱支持物、缓冲至pH 5.0的移动的相溶液的离子强度梯度和在210 nm处操作的UV检测器。复制和定量能力也进行了讨论。该方法已被用于在大鼠脑突触体提取物中的N-乙酰天冬氨酸和N-乙酰天门冬氨酸肽的初步估计。
Abstract A high performance liquid chromatographic (HPLC) procedure for the rapid separation of N-acetylaspartic acid, N-acetyl-aspartyl-glutamic acid and N-acetylaspartyl-glutamyl-aspartic acid is described. The procedure utilizes a pellicular ion-exchange column support, and ionic strength gradient with mobile phase solutions buffered to pH 5.0 and a UV detector operated at 210 nm. Reproducibility and quantitative capabilities are also discussed. The method has been used for a tentative estimation of N-acetylaspartic acid and N-acetylaspartyl peptides in a rat brain synaptosomal extract.