Biodiversity and degradation potential of oil-degrading bacteria isolated from deep-sea sediments of South Mid-Atlantic Ridge
Biodiversity and degradation potential of oil-degrading bacteria isolated from deep-sea sediments of South Mid-Atlantic Ridge
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南大西洋中脊深海沉积物中石油降解细菌的生物多样性和降解潜力
DOI：
10.1016/j.marpolbul.2015.05.065
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发表时间：
2015-08-15
影响因子：
5.8
通讯作者：
Zheng, Li
中科院分区：
文献类型：
--
作者：
Gao, Xiangxing;Gao, Wei;Zheng, Li
以原油为唯一碳源和能源，从大西洋中脊南部深海沉积物中富集了土著石油降解菌群MARA和MARB。生物多样性和群落分析表明，α-变形菌门的成员是MARA财团的主要参与者，而γ-变形菌门的成员是MARB财团的主要参与者，MiSeq测序方法对它们进行了研究。重量法测得20 d后MARA和MARB的石油降解率分别为63.4%和85.8%。分离得到11株不同形态的可培养石油降解菌。根据16 S rRNA基因序列将这些菌株鉴定为Alcanivorax、Bacillus、Dietzia、Erythrobacter、Marinobacter、Mtratireductor和Oceanicola。属于迪茨氏菌属的3个菌株表现出最高的石油降解能力。结果表明，南部MAR沉积物中存在土著微生物群落对石油污染物的固有生物降解能力。(C)2015爱思唯尔有限公司版权所有。
The indigenous oil-degrading bacterial consortia MARA and MARB were enriched from the deep-sea sediments of South Mid-Atlantic Ridge (MAR) with crude oil as sole carbon and energy sources. Biodiversity and community analyses showed that members of alpha-Proteobacteria were the key players in consortium MARA, whereas those of gamma-Proteobacteria were the key players in consortium MARB, which were studied by MiSeq sequencing method. Gravimetric method estimated the oil degradation rates of MARA and MARB to be 63.4% and 85.8%, respectively, after 20 d. Eleven cultivable oil degraders with different morphologies were isolated. These strains were identified as Alcanivorax, Bacillus, Dietzia, Erythrobacter, Marinobacter, Mtratireductor, and Oceanicola based on 16S rRNA gene sequences. Three strains belonging to Dietzia exhibited the highest oil degradation capability. Results indicated that the intrinsic biodegradation capacity of oil contaminants by indigenous microbial communities exists in South MAR sediments. (C) 2015 Elsevier Ltd. All rights reserved.