Dietary Fructose and Microbiota-Derived Short-Chain Fatty Acids Promote Bacteriophage Production in the Gut Symbiont Lactobacillus reuteri
Dietary Fructose and Microbiota-Derived Short-Chain Fatty Acids Promote Bacteriophage Production in the Gut Symbiont Lactobacillus reuteri
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DOI：
10.1016/j.chom.2018.11.016
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发表时间：
2019-02-13
影响因子：
30.3
通讯作者：
van Pijkeren, Jan-Peter
中科院分区：
文献类型：
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作者：
Oh, Jee-Hwan;Alexander, Laura M.;van Pijkeren, Jan-Peter
哺乳动物的肠道包含一个复杂的微生物生态系统，其中有许多溶原菌，溶原菌是在其基因组中插入休眠噬菌体（原噬菌体）的细菌。大约一半的肠道病毒来自溶原菌，这表明这些细菌遇到了促进噬菌体产生的触发器。我们表明，肠道共生菌罗伊氏乳杆菌的噬菌体在胃肠道转运过程中被激活，并且噬菌体的产生在富含果糖的饮食中进一步增加。果糖和暴露于短链脂肪酸激活了参与产生乙酸的Ack途径，这反过来又触发了促进噬菌体产生的细菌应激反应。L. Ack途径或RecA（一种应激反应组分）的罗伊氏突变体表现出减少的噬菌体产生。因此，在肠道共生体中的噬菌体可以被饮食和受饮食影响的代谢产物诱导，这为饮食对肠道噬菌体群落的影响提供了潜在的机制解释。
The mammalian intestinal tract contains a complex microbial ecosystem with many lysogens, which are bacteria containing dormant phages (prophages) inserted within their genomes. Approximately half of intestinal viruses are derived from lysogens, suggesting that these bacteria encounter triggers that promote phage production. We show that prophages of the gut symbiont Lactobacillus reuteri are activated during gastrointestinal transit and that phage production is further increased in response to a fructose-enriched diet. Fructose and exposure to short-chain fatty acids activate the Ack pathway, involved in generating acetic acid, which in turn triggers the bacterial stress response that promotes phage production. L. reuteri mutants of the Ack pathway or RecA, a stress response component, exhibit decreased phage production. Thus, prophages in a gut symbiont can be induced by diet and metabolites affected by diet, which provides a potential mechanistic explanation for the effects of diet on the intestinal phage community.