Effect of Electron Beam Irradiation on Biomechanical Properties of Patellar Tendon Allografts in Anterior Cruciate Ligament Reconstruction
Effect of Electron Beam Irradiation on Biomechanical Properties of Patellar Tendon Allografts in Anterior Cruciate Ligament Reconstruction
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DOI：
10.1177/0363546509361161
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发表时间：
2010-06-01
影响因子：
4.8
通讯作者：
Scheffler, Sven
中科院分区：
文献类型：
--
作者：
Hoburg, Arnd T.;Keshlaf, Salahedeen;Scheffler, Sven
背景：同种异体前交叉韧带的消毒是防止疾病传播的重要前提。然而，目前的大多数灭菌方法都会影响生物组织的力学性能，因此很难实现同种异体移植物的灭菌。假设/目的：本研究的目的是评价新型电子束灭菌方法在不同照射剂量下对人同种异体膝腱移植物生物力学性能的影响。研究设计：对照实验室研究。方法：将32块10 mm宽的人自体骨-髌腱-骨移植物随机分为4组，分别接受15、25、34kGy射线辐照灭菌。于术后即刻评价移植物的生物力学性能。未灭菌的移植物作为对照。结果：两组间的应变和循环伸长响应无显著差异。除34kGy射线外，电子束照射对移植物的硬度和破坏载荷均无显著影响，破坏载荷(1300.6+/-229.2 N)较未灭菌移植物(1630.5+/-331.1 N)显著降低。结论：电子束对同种异体膝腱移植物的体外力学性能影响最小，是一种较理想的灭菌方法。未来的研究将必须评估该过程对同种异体移植物生物学特性的影响。临床意义：电子束照射同种异体膝腱移植物的末端灭菌可以确保移植物更高的安全性，从而提高患者的安全性和接受性。
Background: Sterilization of anterior cruciate ligament (ACL) allografts is an important prerequisite to prevent disease transmission. However, mechanical tissue properties are compromised by most current sterilization procedures, so that uncompromised sterilization of allografts is difficult to achieve.Hypothesis/Purpose: The aim of this study was to evaluate the effect of the novel electron beam sterilization procedure on the biomechanical properties of human patellar tendon allografts at various irradiation dosages. Electron beam sterilization may be an appropriate alternative to gamma sterilization.Study Design: Controlled laboratory study.Methods: Thirty-two human 10-mm wide bone-patellar tendon-bone grafts were randomized into 4 groups of sterilization with 15, 25, or 34 kGy of electron beam irradiation, respectively. The grafts' biomechanical properties were evaluated at time zero. Unsterilized grafts functioned as controls. Biomechanical properties were analyzed during cyclic and load-to-failure testing.Results: Strain and cyclic elongation response showed no significant differences between the groups. Electron beam irradiation had no significant effect on stiffness and failure load with the exception of 34 kGy, which resulted in a significant decrease in failure load (1300.6 +/- 229.2 N) compared with unsterilized grafts (1630.5 +/- 331.1 N).Conclusion: This study showed that electron beam might be an appropriate alternative in sterilization of patellar tendon allografts with minimal effect on mechanical properties of tendon grafts in vitro. Future studies will have to evaluate the effect of the process on the biological properties of allografts in vitro and in vivo.Clinical Relevance: Terminal sterilization of patellar tendon allografts with electron beam irradiation can ensure higher safety of transplanted grafts and hence improve patient safety and acceptance.