Assessing the impact of zero-valent iron (ZVI) nanotechnology on soil microbial structure and functionality: A molecular approach
Assessing the impact of zero-valent iron (ZVI) nanotechnology on soil microbial structure and functionality: A molecular approach
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DOI：
10.1016/j.chemosphere.2011.11.041
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发表时间：
2012-02-01
期刊：
影响因子：
8.8
通讯作者：
Martin, M.
中科院分区：
文献类型：
--
作者：
Fajardo, C.;Ortiz, L. T.;Martin, M.
在这项工作中，纳米级零价铁（NZVI）颗粒被用作固定策略，以降低污染土壤中铅和锌的有效性和流动性。以34 mg g（-1）的剂量将NZVI施用于两种土壤微观环境（MPb和MZn），有效地固定了25%的Pb和20%的zn。NZVI对土壤微生物细胞活力和生物活性影响不大。三个细菌基因（narG， nirS和gyrA）被用作治疗相关的生物标志物。这些生物标志物排除了对大量土壤微生物群落的广泛杀菌作用。转录组分析显示，在NZVI处理后，这些基因的表达率没有任何变化：MPb微观环境中的表达率为1.6 （narG）、0.8 （nirS）和0.7 (gyrA)， MZn微观环境中的表达率为0.6 （narG）、1.2 （nirS）和0.5 （gyrA）。然而，通过荧光原位杂交检测到土壤细菌种群的结构和组成发生了显著变化。因此，我们的研究结果表明，NZVI的毒性可能是高度剂量和物种依赖的，并且所提出的分子方法在评估这种固定策略对土壤微生物种群的影响方面具有有效的适用性。(C) 2011 Elsevier Ltd.版权所有。
In this work, nanoscale zero-valent iron (NZVI) particles have been used as an immobilisation strategy to reduce Pb and Zn availability and mobility in polluted soils. The application of NZVI to two soil microcosms (MPb and MZn) at a dose of 34 mg g(-1) soil efficiently immobilised Pb (25%) and zinc (20%). Exposure to NZVI had little impact on the microbial cellular viability and biological activity in the soils. Three bacterial genes (narG, nirS and gyrA) were used as treatment-related biomarkers. These biomarkers ruled out a broad bactericidal effect on the bulk soil microbial community. A transcriptome analysis of the genes did not reveal any changes in their expression ratios after the NZVI treatment: 1.6 (narG), 0.8 (nirS) and 0.7 (gyrA) in the MPb microcosm and 0.6 (narG), 1.2 (nirS) and 0.5 (gyrA) in the MZn microcosm. However, significant changes in the structure and composition of the soil bacteria population were detected by fluorescence in situ hybridisation. Thus, our results showed that NZVI toxicity could be highly dose and species dependent, and the effective applicability of the proposed molecular approach in assessing the impact of this immobilisation strategy on soil microbial population. (C) 2011 Elsevier Ltd. All rights reserved.