Localization of pectic galactan in tomato cell walls using a monoclonal antibody specific to (1->4)-beta-D-galactan
Localization of pectic galactan in tomato cell walls using a monoclonal antibody specific to (1->4)-beta-D-galactan
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DOI：
10.1104/pp.113.4.1405
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发表时间：
1997-04-01
期刊：
影响因子：
7.4
通讯作者：
Knox, JP
中科院分区：
文献类型：
--
作者：
Jones, L;Seymour, GB;Knox, JP
为了开发果胶中性侧链的抗体探针，产生了从番茄（Lycopersicon esculentum）果皮细胞壁分离的果胶半乳聚糖和（1->4）-β-半乳糖四糖-牛血清白蛋白新糖蛋白的抗血清。这两种抗血清在免疫化学测定和免疫定位研究中的使用表明它们具有非常相似的特异性。分离单克隆抗体（LM 5），并在用新糖蛋白免疫后进行表征。半抗原抑制研究表明，该抗体特异性识别（1->4）-β-半乳糖残基的三个以上连续单元。用抗半乳聚糖抗体对番茄果皮和叶柄细胞壁中果胶的半乳聚糖侧链进行了免疫定位。虽然LM 5表位发生在番茄果实的大多数细胞壁中，但它不存在于腔室凝胶和表皮和亚表皮细胞中。此外，与其他抗果胶抗体相比，LM 5不标记番茄叶柄厚角组织的细胞壁增厚。
To develop antibody probes for the neutral side chains of pectins, antisera were generated to a pectic galactan isolated from tomato (Lycopersicon esculentum) pericarp cell walls and to a (1-->4)-beta-galactotetraose-bovine serum albumin neoglycoprotein. The use of these two antisera in immunochemical assays and immunolocalization studies indicated that they had very similar specificities. A monoclonal antibody (LM5) was isolated and characterized subsequent to immunization with the neoglycoprotein. Hapten inhibition studies revealed that the antibody specifically recognized more than three contiguous units of (1-->4)-beta-galactosyl residues. The anti-galactan antibody was used to immunolocalize the galactan side chains of pectin in tomato fruit pericarp and tomato petiole cell walls. Although the LM5 epitope occurs in most cell walls of the tomato fruit, it was absent from both the locular gel and the epidermal and subepidermal cells. Furthermore, in contrast to other anti-pectin antibodies, LM5 did not label the cell wall thickenings of tomato petiole collenchyma.