Morphological alterations of all stages of spermatogenesis and acrosome reaction in Chinese mitten crab Eriocheir sinensis
Morphological alterations of all stages of spermatogenesis and acrosome reaction in Chinese mitten crab Eriocheir sinensis
复制标题
DOI：
10.1007/s00441-014-2092-5
复制
发表时间：
2015-02
影响因子：
3.6
通讯作者：
Yuanli Wang;Wen-juan Sun;Lin He;Qing Li;Qun Wang
中科院分区：
文献类型：
--
作者：
Yuanli Wang;Wen-juan Sun;Lin He;Qing Li;Qun Wang
中华绒螯蟹（Eriocheirsinensis）是一种季节性繁殖的蟹类，其生殖系统由对称的睾丸、输精管、精囊、附属腺和射精管组成。成熟雄性睾丸的组织学检查显示，在12月至次年4月期间，性腺退化并抑制精子发生过程，在6月至7月期间性腺再生，在8月至10月期间发生最高水平的精子发生。显微镜评估和苏木精和伊红（H&E）染色用于描述所有的精子发生阶段（精原细胞，初级和次级精母细胞，精子细胞和精子）。为了观察精子发生过程中的形态学变化，我们成功地用大肠杆菌睾丸组织进行了原代细胞培养。sinensis的克隆，为进一步研究河蟹细胞的永生化奠定了基础。在精子与卵母细胞相互作用过程中，精子发生一系列终末形态学变化，称为顶体反应（AR）。本研究还提供了一个详细的描述的顶体反应的结构改变的E。顶体复合体和杯状核分别位于精子的前部和后部。雄性生殖细胞的发育涉及一系列严格控制的分化开关。本研究的目的是增加我们对精子发生过程中的形态学变化和顶体反应的认识，顶体反应的变化是E.中国产。
The Chinese mitten crab (Eriocheir sinensis) is a seasonally breeding species and its reproductive system comprises paired symmetrical structures: testes, vasa deferentia, seminal vesicles, accessory glands and ejaculatory ducts. Histological examination of the testis of mature males reveals regression of the gonads and inhibition of the process of spermatogenesis during December to April of the following year, the regeneration of the gonads during June to July and the occurrence of the highest level of spermatogenesis during August to October. Microscopic assessments and hematoxylin and eosin (H&E) staining were used to describe all spermatogenic stages (spermatogonia, primary and secondary spermatocytes, spermatids and spermatozoids). To observe the morphological changes during spermiogenesis, we successfully initiated primary cell culture using testis tissue ofE. sinensis, which will lay a solid foundation for further work on the immortalization of crab cells. During the interaction between the sperm and oocyte, the fertilizing spermatozoon must undergo a series of terminal morphological changes, called the acrosome reaction (AR). This study also provides a detailed description of the structural alterations of the acrosome reaction ofE. sinensis.The acrosome complex and cup-shaped nucleus are located at the anterior and posterior of the spermatozoon, respectively. Male germ cell development involves a tightly controlled sequence of differentiation switches. The purpose of this study is to increase our knowledge of the morphological alterations during spermatogenesis and the acrosome reaction, whose changes are a fundamental requirement for fertilization ofE. sinensis.