Opiate analgesics inhibit substance P release from rat trigeminal nucleus
Opiate analgesics inhibit substance P release from rat trigeminal nucleus
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DOI：
10.1016/0304-3959(79)90061-7
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发表时间：
1977-08
期刊：
影响因子：
64.8
通讯作者：
T. Jessell;L. Iversen
中科院分区：
文献类型：
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作者：
T. Jessell;L. Iversen
一种新的技术已经被开发出来，用于从自由行走的猫的个体初级传入神经元群中稳定地、长期地记录。通过腰椎的一个小的椎板切开术，将一些细小的、柔软的钢丝插入到脊神经根神经节中。每根导线的断端可记录到1~3个背根神经节神经元的稳定可分离的动作电位，每个单位保持1~4天。在运动过程中，我们研究了大范围的有髓躯体感觉传入(传导速度为30-120米/秒)。大多数被研究的皮肤和本体感觉传入都是步进周期成分复杂组合的敏感监测器，它们的放电模式通常很难从现有信息中预测出来。这项技术也可用于清醒动物的疼痛研究。鸦片镇痛剂抑制大鼠三叉神经核P物质的释放。-TM Jessell和LL Iversen，Natural(Lond.)，268(1977)540-551描述了阿片受体结合和P物质(SP)在大鼠脑干和脊髓中的比较分布。本实验观察了大鼠三叉神经脊束核脑片在刺激后释放SP的情况，以寻找影响其初级传入终末释放的因素。阿片类镇痛剂可抑制刺激诱发的SP释放。提示阿片受体位于含SP的初级传入神经末梢的胶状质内，阿片类镇痛剂与钙内流等偶联机制相互作用。
A new technique has been developed for stable, long-term recording from groups of individual primary afferent neurons in the freely walking cat. A number of fine, flexible wires are inserted into dotal root ganglia via a small laminotomy in the lumbar spine. The cut end of each wire can record stable and separable action potentials from I to 3 dorsal root ganglion neurons; each unit has held for 1--4 days. A broad range of myelinated somatosensory afferents (conduction velocities of 30-120 m/sec) have been studied during locomotion. Most cutaneous and proprioceptive afferents studied have been sensitive monitors of complex combinations of step cycle components, and their firing patterns would often have been difficult to predict from existing information. The technique may be of use also in studies of pain in awake animals.Opiate analgesics inhibit substance P release from rat trigeminal nucleus.-TM Jessell and LL Iversen, Nature (Lond.), 268 (1977) 540--551 Comparative distribution of opiate receptor binding and of substance P (SP) in the rat brain stem and spinal cord is described. The stimulus evoked release of SP from rat spinal trigeminal nucleus slices in vitro was examined to find the factors which control its release from primary afferent terminals. Opiate analgesics were found to suppress the stimulus-evoked release of SP. It is suggested that some opiate receptors are located in the substantia gelatinosa on primary afferent nerve terminals containing SP and that opiate analgesics interact with coupling mechanism, eg calcium influx.