HOM HOX TYPE HOMEOBOXES IN THE CHELICERATE LIMULUS-POLYPHEMUS
HOM HOX TYPE HOMEOBOXES IN THE CHELICERATE LIMULUS-POLYPHEMUS
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DOI：
10.1006/mpev.1993.1019
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发表时间：
1993-09-01
影响因子：
4.1
通讯作者：
BUSS, LW
中科院分区：
文献类型：
--
作者：
CARTWRIGHT, P;DICK, M;BUSS, LW
应用聚合酶链反应技术对北美鲎HOM/Hox型同源盒基因进行了研究。结果表明，有28个同源盒片段与果蝇的HOM-C序列和小鼠的Hox B序列具有44-80%的核苷酸序列相似性，根据氨基酸序列同源性确定，鲎有1 - 4个代表HOM/Hox同源物。一个包括鲎基因序列和同源小鼠Hox B和果蝇HOM-C序列的简约性分析揭示了鲎基因盒与果蝇或小鼠基因作为姐妹分支的单系分组的几个实例。在现有的HOM/Hox团簇演化模型下，这些结果表明Limulus有多达4个HOM/Hox型团簇。这是第一个表明在非脊椎动物分类群中存在多个簇的证据。鲎中小鼠Hoxb-3的推定同源物表明该基因存在于节肢动物和脊椎动物的共同祖先中，而在果蝇中丢失。
The polymerase chain reaction was used to survey HOM/Hox type homeobox genes in the North American horseshoe crab,Limulus polyphemus. The survey reveals 28 different homeobox fragments possessing 44-80% nucleotide sequence similarity with HOM-C sequences inDrosophilaand Hox B sequences in mouse.Limulushas one to four representatives of HOM/Hox cognates when fragments are assigned according to amino acid identity. A parsimony analysis including theLimulussequences and homologous mouse Hox B andDrosophilaHOM-C sequences reveals several instances of monophyletic groupings ofLimulushomeoboxes with aDrosophilaor mouse gene as the sister branch. Under the current model of HOM/Hox cluster evolution, these results suggest thatLimulushas as many as four HOM/Hox type clusters. This is the first evidence suggesting the presence of multiple clusters in a nonvertebrate taxon. Putative homologs of mouseHoxb-3 inLimulussuggest that the gene was present in the common ancestor of arthropods and vertebrates and has been lost inDrosophila.