Programmed cell death of secretory cavity cells in fruits of Citrus grandis cv. Tomentosa is associated with activation of caspase 3-like protease
Programmed cell death of secretory cavity cells in fruits of Citrus grandis cv. Tomentosa is associated with activation of caspase 3-like protease
复制标题
柑橘果实中分泌腔细胞的程序性细胞死亡。
DOI：
10.1007/s00468-012-0752-1
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发表时间：
2012-12-01
影响因子：
2.3
通讯作者：
Wu, Hong
中科院分区：
文献类型：
--
作者：
Liu, Peiwei;Liang, Shejian;Wu, Hong
在此之前，我们发现在柑橘分泌腔发育过程中，分泌细胞的降解通常通过程序性细胞死亡发生。（奥斯贝克）。这一发现表明，分泌腔可以作为一种新的细胞生物学模型来研究植物细胞程序性死亡的调控机制。为进一步研究柑桔果实分泌腔发育过程中的细胞程序性死亡，以沙田柚（Citrus grandis cv.托门托萨使用光学显微镜和电子显微镜TUNEL检测，免疫组织化学和免疫细胞化学，我们描述了精确的空间和时间的变化，在caspase 3样分布，染色质凝聚和DNA片段化过程中的程序性细胞死亡的分泌腔细胞。Caspase 3样蛋白在程序性细胞死亡的分泌腔细胞的胞浆和胞核中均有表达，与染色质浓缩和DNA断裂密切相关。有趣的是，在核仁中检测到caspase 3样和DNA片段化。我们的研究结果表明，caspase 3样蛋白可能参与分泌腔细胞的程序性细胞死亡，特别是染色质浓缩、DNA片段化、核降解和某些细胞器的降解。
Previously, we found that secretory cell degradation typically occurred through programmed cell death during secretory cavity development in Citrus sinensis L. (Osbeck). This finding indicated that secretory cavities could be utilized as a new cell biology model for investigating the regulatory mechanisms of plant programmed cell death. To study further the programmed cell death during secretory cavity development in Citrus fruit, we studied the morphogenetic characteristics of secretory cavities during their development in Citrus grandis cv. Tomentosa. Using light microscope- and electron microscope-TUNEL assays, immunohistochemistry and immunocytochemistry, we described the precise spatial and temporal alterations in caspase 3-like distribution, chromatin condensation and DNA fragmentation during the programmed cell death of secretory cavity cells. Caspase 3-like was found to be significantly located in both the cytoplasm and the nucleus of secretory cavity cells undergoing programmed cell death, and caspase 3-like is closely associated with chromatin condensation and DNA fragmentation. Interestingly, both caspase 3-like and DNA fragmentation were detected in the nucleoli. Our findings suggest that caspase 3-like may be involved in the programmed cell death of secretory cavity cells, especially in chromatin condensation, DNA fragmentation, nuclear degradation and the degradation of certain organelles.