A pilot study of new promising non-coding RNA diagnostic biomarkers for early-stage colorectal cancers
A pilot study of new promising non-coding RNA diagnostic biomarkers for early-stage colorectal cancers
复制标题
早期结直肠癌新的有前景的非编码 RNA 诊断生物标志物的初步研究
DOI：
10.1515/cclm-2019-0052
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发表时间：
2019-07-01
影响因子：
6.8
通讯作者：
Zhang, Xiaoren
中科院分区：
文献类型：
--
作者：
Liu, Hanshao;Ye, Deji;Zhang, Xiaoren
摘要背景用于检测结直肠癌（CRC）的诊断生物标志物缺乏。最近的研究表明，循环中的长链非编码RNA具有作为检测癌症的生物标志物的潜力。分析lncRNA91H、PVT-1和MEG3在结直肠癌检测中的意义。方法检测18例结直肠癌患者和20例正常对照血浆中13种候选lncRNA的表达水平。然后，我们通过确定六种有希望的lncRNA在CRC组织和正常结直肠组织中的表达水平来验证我们的发现。最后，我们评估了58名CRC患者和56名非癌对照血浆中lncRNA 91H、PVT-1和MEG3的临床相关性。结果大肠癌患者血浆中lncRNA91H、PVT-1和MEG3的表达水平显著高于正常对照组。91 H、PVT-1和MEG3的组合可以区分CRC患者和非癌对照，接受者操作曲线下面积（AUC）为0.877，临界值为0.3816，灵敏度为82.76%，特异性为78.57%。更重要的是，lncRNA的组合在检测早期CRC中显示出比CEA和CA19 - 9（目前用于CRC检测的生物标志物）的组合更高的灵敏度（p <0.0001）。结论lncRNA 91 H、PVT-1和MEG 3是早期CRC有希望的诊断生物标志物。
Abstract Background Diagnostic biomarkers for the detection of colorectal cancers (CRCs) are lacking. Recent studies have demonstrated that circulating long non-coding RNAs have the potential to serve as biomarkers for the detection of cancers. We analyzed the significance of lncRNAs 91H, PVT-1 and MEG3 in the detection of CRC. Methods We examined the expression levels of 13 candidate lncRNAs in the plasma of 18 CRC patients and 20 non-cancerous controls. Then, we validated our findings by determining the expression levels of six promising lncRNAs in CRC tissues and normal colorectal tissues. Finally, we evaluated the clinical relevance of lncRNAs 91H, PVT-1 and MEG3 in the plasma of 58 CRC patients and 56 non-cancerous controls. Results Our data revealed that the expression levels of lncRNAs 91H, PVT-1 and MEG3 were significantly higher in plasma samples from CRC patients than in those from non-cancerous controls. The combination of 91H, PVT-1 and MEG3 could discriminate CRC patients from non-cancerous controls with an area under the receiver-operating curve (AUC) of 0.877 at a cut-off value of 0.3816, with a sensitivity of 82.76% and 78.57% specificity. More importantly, the combination of lncRNAs shows more sensitivity in the detection of early-stage CRC than the combination of CEA and CA19-9, biomarkers currently used for CRC detection (p < 0.0001). Conclusions lncRNAs 91H, PVT-1 and MEG3 are promising diagnostic biomarkers for early-stage CRC.