Evaluation of the Anti-Aging Effects of a Probiotic Combination Isolated From Centenarians in a SAMP8 Mouse Model.
Evaluation of the Anti-Aging Effects of a Probiotic Combination Isolated From Centenarians in a SAMP8 Mouse Model.
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DOI：
10.3389/fimmu.2021.792746
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发表时间：
2021
影响因子：
7.3
通讯作者：
Chen T
中科院分区：
文献类型：
--
作者：
Fang X;Yue M;Wei J;Wang Y;Hong D;Wang B;Zhou X;Chen T
人口老龄化是当今社会一个突出的全球性问题。然而，目前还没有很好的方法来治疗或预防衰老，因此抗衰老研究具有至关重要的意义。本研究从百岁老人中筛选出4株益生菌（发酵乳杆菌SX-0718、乳酸菌L.干酪杆菌SX-1107、长双歧杆菌SX-1326和B。animalis SX-0582）以形成益生菌组合。通过使用衰老加速小鼠倾向8（SAMP 8）模型，通过使用行为测试、神经炎症、肠道炎症和肠道微生物群来评估益生菌组合的抗衰老作用。结果表明，益生菌组合改善了衰老小鼠受损的空间记忆，运动功能障碍，并减少了探索行为。益生菌组合抑制Toll样受体4（TLR 4）/核因子κ B（NFκB）诱导的神经炎症并上调Sirt 1的表达以保护海马神经元。同时，益生菌组合调节肠道微生物群，降低SAMP 8小鼠中Alistipes和Prevotella的相对丰度，抑制TLR 4/NFκ B诱导的肠道炎症，并增加肠道通透性相关蛋白封闭带-1（ZO-1）和Occuldin的表达。益生菌组合的抗衰老作用可能是通过调节肠道微生物群和抑制TLR 4/NFκ B诱导的炎症。本研究为今后益生菌组合的生产和应用提供了依据和技术支持。
Population aging is a prominent global problem in today’s society. However, there are currently no good methods to treat or prevent aging, so anti-aging research has crucial implications. In this research, we screened bacteria from centenarians, and finally selected four probiotics (Lactobacillus fermentum SX-0718, L. casei SX-1107, Bifidobacterium longum SX-1326, and B. animalis SX-0582) to form a probiotic combination. By using the senescence accelerated mouse prone 8 (SAMP8) model, the anti-aging effects of the probiotic combination were evaluated by using behavioural testing, neuroinflammation, intestinal inflammation, and intestinal microbiota. The results showed that probiotic combination improved the impaired spatial memory, motor dysfunction, and decreased exploratory behavior in aging mice. The probiotic combination inhibited Toll-like receptor 4 (TLR4)/nuclear factor kappa B (NFκB)-induced neuroinflammation and up-regulated the expression of Sirt 1 to protect hippocampal neurons. At the same time, the probiotic combination regulated the intestinal microbiota, reduced the relative abundance of Alistipes and Prevotella in SAMP8 mice, inhibited TLR4/NFκB-induced intestinal inflammation, and increased the expression of intestinal permeability related proteins zonula occludens-1 (ZO-1) and Occuldin. The anti-aging effects of the probiotic combination may be through the regulating intestinal microbiota and inhibiting TLR4/NFκB-induced inflammation. This research provides the basis and technical support for the future production and application of the probiotic combination.