CRISPR-Cas and restriction-modification systems are compatible and increase phage resistance
CRISPR-Cas and restriction-modification systems are compatible and increase phage resistance
复制标题
DOI：
10.1038/ncomms3087
复制
发表时间：
2013-07-01
影响因子：
16.6
通讯作者：
Moineau, Sylvain
中科院分区：
文献类型：
--
作者：
Dupuis, Marie-Eve;Villion, Manuela;Moineau, Sylvain
细菌已经形成了一套屏障来保护自己免受噬菌体和质粒核酸等入侵者的侵害。存在不同的原核防御系统，其中至少有两种直接靶向进入的DNA：限制修饰（R-M）和CRISPR-Cas系统。就其本身而言，它们是外来DNA入侵的不完美屏障。在这里，我们表明R-M和CRISPR-Cas系统是相容的，并且通过切割它们各自的靶位点来共同作用以增加细菌细胞的总体噬菌体抗性。此外，我们表明噬菌体DNA的特异性甲基化不会损害CRISPR-Cas的获得或干扰活性。总而言之，两种机制都可以用来减少依赖于细菌生长和/或发酵的过程中的噬菌体污染。
Bacteria have developed a set of barriers to protect themselves against invaders such as phage and plasmid nucleic acids. Different prokaryotic defence systems exist and at least two of them directly target the incoming DNA: restriction-modification (R-M) and CRISPR-Cas systems. On their own, they are imperfect barriers to invasion by foreign DNA. Here, we show that R-M and CRISPR-Cas systems are compatible and act together to increase the overall phage resistance of a bacterial cell by cleaving their respective target sites. Furthermore, we show that the specific methylation of phage DNA does not impair CRISPR-Cas acquisition or interference activities. Taken altogether, both mechanisms can be leveraged to decrease phage contaminations in processes relying on bacterial growth and/or fermentation.