EARLY EPOCHAL MAPS OF 2 DIFFERENT CELL-ADHESION MOLECULES
EARLY EPOCHAL MAPS OF 2 DIFFERENT CELL-ADHESION MOLECULES
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DOI：
10.1073/pnas.80.14.4384
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
THIERY, JP
中科院分区：
文献类型：
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作者：
EDELMAN, GM;GALLIN, WJ;THIERY, JP
N-CAM是一种神经细胞粘附分子，以前已经发现在发育和功能的几个时期表达，首先作为胚胎发生的早期标志物，后来在器官发生期间，最后在成年生活中。利用荧光抗体技术，已将肝细胞粘附分子L-CAM定位于鸡的胚胎和成体组织中。在胚胎早期，L-CAM和N-CAM出现在上胚层和下胚层组织中。此后，L-CAM分布在所有3个胚层中。然而，神经形成的发病，L-CAM消失在该地区的神经板和N-CAM在该地区的数量增加。L-CAM强烈地出现在所有出芽的内胚层结构（肝、胰腺、肺、甲状腺、甲状旁腺、胸腺和腔上囊）上，而N-CAM最强烈地出现在神经板、神经管和心脏中胚层中，但在内胚层衍生物中未发现。在基板中，L-CAM和N-CAM都存在，直到最终的神经结构形成，此时L-CAM消失。在肾前体细胞中，2 CAM遵循复杂的出现和消失的相互模式。然而，在大多数情况下，这2种分子的分布在器官发生过程中并不重叠。与N-CAM一样，L-CAM在成体组织中以独特的表达模式持续存在。在胚胎发育过程中，这两种不同的CAM分布在来源于> 2/3的早期胚胎表面的组织上。解释地图总结CAM分布在一个定义的发展时期提出了一个关键的作用，L-CAM和N-CAM在胚胎诱导。与这种解释相一致，并且与当器官雏形形成时胚层的连续性丧失的事实相一致，CAM都不限于分布于单个胚层。在早期时期的地图中，缺乏L-CAM和N-CAM的区域包括脏层和体层的某些部分。某些源自该侧板中胚层的成体组织如平滑肌也缺乏L-CAM和N-CAM。在这些和类似来源的组织中可能存在至少1个CAM。
N-CAM, the neural cell-adhesion molecule, has previously been found to be expressed during several epochs of development and function, first as an early marker in embryogenesis, later during organogenesis, and finally in adult life. L-CAM, the liver cell-adhesion molecule, has now been localized in embryonic and adult tissues of the chicken by fluorescent antibody techniques. In the early embryonic epoch, L-CAM and N-CAM appeared in epiblastic and hypoblastic tissues. L-CAM was distributed thereafter across all 3 germ layers. By the onset of neurulation, however, L-CAM disappeared in the region of the neural plate and N-CAM increased in amount in that region. L-CAM appeared strongly on all budding endodermal structures (liver, pancreas, lung, thyroid, parathyroid, thymus and bursa of Fabricus) whereas N-CAM appeared most strongly in the neural plate, neural tube and in cardiac mesoderm but was not found in endodermal derivatives. In placodes, both L-CAM and N-CAM were present until the formation of definitive neural structures, at which time L-CAM disappeared. In kidney precursors, the 2 CAM followed a complex reciprocal pattern of appearance and disappearance. For the most part, however, the distributions of the 2 molecules did not overlap during organogenesis. Like N-CAM, L-CAM persisted in a distinctive pattern of expression in adult tissues. During embryonic development, the 2 different CAM were distributed on tissues derived from > 2/3 of the early embryonic surface. Interpretation of maps summarizing CAM distributions over a defined developmental epoch suggested a key role for both L-CAM and N-CAM in embryonic induction. Consistent with this interpretation and with the fact that the continuity of germ layers is lost when organ rudiments are formed, neither of the CAM was limited in distribution to a single germ layer. The regions of the early epochal maps that lacked both L-CAM and N-CAM comprised some portions of the splanchnopleure and somatopleure. Certain adult tissues that derive from this lateral plate mesoderm such as smooth muscle also lacked L-CAM and N-CAM. At least 1 more CAM may exist in these and similarly derived tissues.