Quorum sensing in gram-positive bacteria: assay protocols for staphylococcal agr and enterococcal fsr systems.
Quorum sensing in gram-positive bacteria: assay protocols for staphylococcal agr and enterococcal fsr systems.
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DOI：
10.1007/978-1-4939-0467-9_3
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发表时间：
2014
影响因子：
--
通讯作者：
Akane Shojima;J. Nakayama
中科院分区：
文献类型：
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作者：
Akane Shojima;J. Nakayama
硫内酯/内酯肽介导的群体感应（QS）系统通常用于革兰氏阳性菌中以控制多种表型的表达，包括毒力因子的产生和生物膜的形成。在这里，我们描述了两个代表性的革兰氏阳性病原体，金黄色葡萄球菌和粪肠球菌的研究QS系统（agrandfsr）的检测协议。这些方便的测定系统可用于QS抑制剂的筛选以及用于基础研究以解决这些QS系统的机制。
A thiolactone/lactone peptide-mediated quorum sensing (QS) system is commonly employed in gram-positive bacteria to control the expression of a variety of phenotypes, including the production of virulence factors and biofilm formation. Here, we describe assay protocols for the well-studied QS systems (agrandfsr) of two representative gram-positive pathogens,Staphylococcus aureusandEnterococcus faecalis. These convenient assay systems are useful for the screening of QS inhibitors as well as for basic research to address the mechanism of these QS systems.