Large-Scale Epitope Identification Screen and Its Potential Application to the Study of Alopecia Areata.
Large-Scale Epitope Identification Screen and Its Potential Application to the Study of Alopecia Areata.
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大规模表位鉴定筛选及其在斑秃研究中的潜在应用。
DOI：
10.1016/j.jisp.2017.10.001
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Sette,Alessandro
中科院分区：
文献类型：
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作者：
LindestamArlehamn,CeciliaS;Paul,Sinu;ChunWang,EddyHsi;deJong,Annemieke;Christiano,AngelaM;Sette,Alessandro
人类疾病（例如传染病和自身免疫性疾病）中抗原特异性反应的发现主要利用候选方法。该方法通过不同的分子生物学检测测试了一组在发育或病理网络中具有已知作用的蛋白质或分子是否可以引发疾病表型（例如 T 细胞反应）的假设。尽管这种方法可以通过直接功能测定提供有关疾病的宝贵知识，但由于最初的候选选择过程，它可能会产生偏差。在自身免疫环境中，自身抗原表位的识别通常从验证轶事观察开始，这可能会限制可能引发疾病发作的候选抗原组的范围。因此，需要一种公正的、大规模的筛选和识别方法来补充候选方法，以确保包含所有可能的抗原进行生物验证。近年来，基因组学（下一代 DNA 测序）、蛋白质组学和生物信息学技术的进步，再加上大规模的基于人群的研究，使我们能够进行高通量抗原和表位鉴定，这有可能发展成适用于更广泛人群的治疗策略。
The discovery of antigen-specific responses in human diseases, such as infectious and autoimmune diseases, predominantly utilizes a candidate approach. This approach tests the hypothesis whether a panel of proteins or molecules with known roles in the developmental or pathology network can incite disease phenotype such as T-cell response via different molecular biology assays. Although this approach can provide valuable knowledge of the disease through direct functional assays, it can be biased due to the initial candidate selection process. In an autoimmune setting, identification of autoantigen epitopes usually began with validation anecdotal observations that potentially limit the scope of the candidate antigen panel that could initiate disease onset.As such, an unbiased, large-scale screening and identification method is needed to complement the candidate approach to ensure the inclusion of all possible antigens for biological validation. The advancement of technologies in genomics (next generation DNA sequencing), proteomics, and bioinformatics in conjunction with large population-based studies in recent years now allows us to perform high-throughput antigen and epitope identification that could potentially develop into therapeutic strategies that can apply to a wider population.