A simple method for the construction of small format tissue arrays
A simple method for the construction of small format tissue arrays
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DOI：
10.1136/jcp.56.2.144
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发表时间：
2003-02-01
影响因子：
3.4
通讯作者：
Salcedo, M
中科院分区：
文献类型：
--
作者：
Hidalgo, A;Piña, P;Salcedo, M
组织阵列可以并行评估大量样品中的分子靶标。阵列的构建带来了技术上的困难：而且，组织阵列是昂贵的，特别是对于中小型实验室。本报告描述了一种方法；用于使用广泛可用的材料构建36个样品阵列。一个钝化的16提取石蜡瓶，并在空白石蜡块上手动定义一个6 × 6的矩阵。用14号针将36个包埋癌前病变和浸润性宫颈癌的石蜡组织芯注射到基质中。使用标准切片机对该组织进行切片，并用于CD44变体9和白细胞介素18的免疫检测，结果令人满意。这种方法可以应用于任何实验室，而不需要专门的设备，为组织阵列的广泛应用提供了一个很好的选择。
Tissue arrays can evailuate molecular targets in high numbers of samples in parallel,. Array construction presents technical difficulties :and, tissue arrayers are expensive, particularly for small and medium sized laboratories. This report describes a method; for the construction of 36 sample arrays using widely available materials. A blunted 16 extract paraffin wax cylinders and manually define a 6 x 6 matrix on a blank paraffin wax block. Tissue cores from 36 paraffin wax embedded premalignant lesions and invasive cervical carcinomas were, injected into the matrix using a 14 gauge needle. This tissue, array was sectioned using a standard microtome and used for the immunodetection of CD44 variant 9 and interleukin 18 with satisfactory results. This method can be applied in any laboratory, without the need of specialised equipment, offering a good alternative for the wider application of tissue arrays.