Grid Cells in Mice
Grid Cells in Mice
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DOI：
10.1002/hipo.20472
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发表时间：
2008-01-01
期刊：
影响因子：
3.5
通讯作者：
Moser, May-Britt
中科院分区：
文献类型：
--
作者：
Fyhn, Marianne;Hafting, Torkel;Moser, May-Britt
内侧内嗅皮层（EC）是大鼠自我定位表征神经网络的一部分。该系统的关键细胞类型是网格细胞，其多个放电场以非常规则的三角形或六边形模式跨越环境。网格细胞和其他细胞类型的基本特性已经被描述，但负责位置代码形成和维持的神经元机制仍然难以捉摸。这些机制可以通过基因干预策略来研究，其中内嗅-海马网络的特定成分被激活或沉默。由于基因敲除小鼠通常用于这种有针对性的干预，我们想知道网格活动是否也在小鼠中表达。小鼠内侧外皮层浅层主要神经元具有与大鼠相似的稳定网格场。相邻的网格单元共享相同的间距和方向，但具有不同的空间相位，因此少量网格单元共同代表环境中的所有位置。网格的间距随着距离内侧脑脊液背缘的距离而增加。在背端记录的栅格间距的最低值与大鼠的栅格间距相当，这表明栅格场并不与身体大小成比例地扩大。与大鼠一样，网格细胞与头向细胞和连接位置x头向细胞共定位。网格细胞在小鼠中的演示为内嗅-海马网络的转基因分析奠定了基础。(C) 2008 Wiley-Liss, Inc。
The medial entorhinal cortex (EC) is a part of the neural network for the representation of self-location in the rat. The key cell type of this system is the grid cell, whose multiple firing fields span the environment in a remarkably regular triangular or hexagonal pattern. The basic properties of grid cells and other cell types have been described, but the neuronal mechanisms responsible for the formation and maintenance of the place code remain elusive. These mechanisms can be investigated by genetic intervention strategies, where specific components of the entorhinal-hippocampal network are activated or silenced. Because of the common use of knockout mice for such targeted interventions, we asked if grid activity is expressed also in the mouse. Principal neurons in the superficial layers of mouse medial EC had stable grid fields similar to those of the rat. Neighboring grid cells shared a common spacing and orientation but had a different spatial phase, such that a small number of grid cells collectively represented all locations in the environment. The spacing of the grid increased with distance from the dorsal border of the medial EC. The lowest values for grid spacing, recorded at the dorsal end, were comparable to those of the rat, suggesting that grid fields do not scale up proportionally with body size. Grid cells were colocalized with head-direction cells and conjunctive place x head-direction cells, as in the rat. The demonstration of grid cells in mice prepares the ground for transgenic analyses of the entorhinal-hippocampal network. (C) 2008 Wiley-Liss, Inc.