Treatment of chondral defects of the knee with one step matrix-assisted technique enhanced by autologous concentrated bone marrow: In vitro characterisation of mesenchymal stem cells from iliac crest and subchondral bone
Treatment of chondral defects of the knee with one step matrix-assisted technique enhanced by autologous concentrated bone marrow: In vitro characterisation of mesenchymal stem cells from iliac crest and subchondral bone
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DOI：
10.1016/j.injury.2010.09.027
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发表时间：
2010-11-01
影响因子：
2.5
通讯作者：
Volpi, Piero
中科院分区：
文献类型：
--
作者：
de Girolamo, Laura;Bertolini, Giulia;Volpi, Piero
软骨修复仍然是一个未解决的问题。近年来，人们提出了许多基于细胞的治疗方法，以获得软骨缺损的良好再生。自体基质诱导软骨形成技术 (AMIC (R)) 将微骨折手术与特定生物膜的使用相结合。对接受 AMIC (R) 技术治疗的患者的髂嵴和膝关节软骨下骨中采集的骨髓细胞群的表型特征进行分析，并通过自体浓缩骨髓增强，以评估细胞群的潜在变异。 11 名患有 III 级或 IV 级孤立软骨损伤的患者样本接受了 AMIC (R) 技术的治疗，并通过使用自体浓缩骨髓进行了增强。通过 FACS 分析对来自髂嵴和所产生的微骨折的一小部分骨髓样本进行分析，然后进行培养以验证其增殖和分化潜力。从髂嵴采集的浓缩骨髓细胞中平均有 0.04% 呈现间充质干细胞表型（CD34 /CD45（低）/CD271（高）），而这些细胞中只有 0.02% 被鉴定出从膝盖微骨折过程中采集的样本中获得。经过两次培养传代后，细胞表达了 MSC 的特殊特征。只有来自骨髓的间充质干细胞才能长期增殖并能够在培养物中有效分化。尽管AMIC（R）方法有很多优点，但微骨折技术应用中的手术技术仍然至关重要，并影响最终结果。 (C) 2010 Elsevier Ltd. 保留所有权利。
Cartilage repair is still an unsolved problem. In the last years many cell-based treatments have been proposed, in order to obtain good regeneration of cartilage defects. The Autologous Matrix-Induced Chondrogenesis technique (AMIC (R)) combines the micro-fracture procedure with the use of a specific biological membrane.The phenotypic feature of bone marrow cell population, harvested from iliac crest and knee subchondral bone of patients treated with the AMIC (R) technique, enhanced by autologous concentrated bone marrow, was analysed to evaluate potential variations of the cell population. Samples of eleven patients, with isolated chondral lesions grade III or IV were treated with the AMIC (R) technique, enhanced by the use of autologous concentrated bone marrow. A small fraction of bone marrow samples, both from iliac crest and from the created micro-fractures, was analysed by FACS analysis and then cultured to verify their proliferative and differentiation potential.An average of 0.04% of concentrated bone marrow cells harvested from the iliac crest, presented mesenchymal stem cell phenotype (CD34 /CD45(low)/CD271(high)), whereas just 0.02% of these cells were identified from the samples harvested during the creation of micro-fractures at the knee. After two passages in culture, cells expressed a peculiar profile for MSC. Only MSC from bone marrow could be long-term propagated and were able to efficiently differentiate in the cultures. Although the AMIC (R) approach has many advantages, the surgical technique in the application of the microfracture technique remains essential and affects the final result. (C) 2010 Elsevier Ltd. All rights reserved.