Effects of sulfadiazine-contaminated fresh and stored manure on a soil microbial community
Effects of sulfadiazine-contaminated fresh and stored manure on a soil microbial community
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DOI：
10.1016/j.ejsobi.2010.10.004
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发表时间：
2011-01-01
影响因子：
4.2
通讯作者：
Thiele-Bruhn, Soeren
中科院分区：
文献类型：
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作者：
Hammesfahr, Ute;Kotzerke, Anja;Thiele-Bruhn, Soeren
土壤微生物对被兽用抗生素污染的粪便的反应还不清楚。为此，通过57 d的培养试验，研究了磺胺嘧啶（SDZ）污染粪肥对土壤微生物的影响。粪便可以直接从给药的猪身上获得，也可以随后储存六个月。我们假设SDZ污染的粪便改变了土壤微生物的功能和结构组成，粪便储存减少了抗生素的作用。潜在的硝化和氮矿化率以及磷脂脂肪酸（PLFA）模式进行了测定。添加未受污染的粪肥影响土壤中的氮循环过程和微生物群落结构，新鲜和储存的粪肥之间的影响不同。潜在的硝化，氨化和氮矿化率和比例的细菌/真菌和革兰氏阴性/革兰氏阳性菌来自PLFA处理的土壤中存储相比，新鲜的，未受污染的粪便。受SDZ污染的粪便对氮循环过程的影响因粪便储存而略有差异，而PLFA（tot）和衍生的细菌/真菌比仅因储存的受污染粪便而降低。SDZ降低硝化和氮矿化速率，而氨化增加。SDZ对土壤微生物的影响是显着的，但SDZ的生物有效性部分（氯化钙提取）迅速下降。我们的结论是，传统的粪肥储存是不适合的，以减少磺胺类抗生素在土壤环境中的风险。(C)2010年Elsevier Masson SAS。All rights reserved.
The response of soil microorganisms to manure contaminated by veterinary antibiotics is not well understood. Therefore, a 57-d incubation experiment was performed to investigate effects of sulfadiazine (SDZ) contaminated manure on soil microorganisms. Manure was either obtained directly from medicated pigs or subsequently stored for six month. We hypothesized that SDZ-contaminated manure changes functions and structural composition of soil microorganisms and that manure storage reduces antibiotic effects. Rates of potential nitrification and N mineralization as well as PLFA (phospholipid fatty acid) patterns were determined. Addition of uncontaminated manure to soil affected N cycle processes and the microbial community structure, and effects differed between fresh and stored manure. The rates of potential nitrification, ammonification and N mineralization and ratios of bacteria/fungi and gram-negative/gram-positive bacteria derived from PLFA were lower in soil treated with stored compared to fresh, uncontaminated manure. SDZ-contaminated manure affected N cycle processes with slight differences due to manure storage, whereas PLFA(tot) and the derived bacteria/fungi ratio were only reduced by stored, contaminated manure. SDZ reduced rates of nitrification and N mineralization, while ammonification increased. Effects of SDZ on soil microorganisms were significant although the bioavailable SDZ fraction (CaCl2-extractable) rapidly declined. We conclude that conventional manure storage is unsuited to reduce risks from sulfonamide antibiotics in the soil environment. (C) 2010 Elsevier Masson SAS. All rights reserved.