Developmental changes of CD34 expression by murine hematopoietic stem cells
Developmental changes of CD34 expression by murine hematopoietic stem cells
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DOI：
10.1016/s0301-472x(00)00535-x
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发表时间：
2000-11-01
影响因子：
2.6
通讯作者：
Ogawa, M
中科院分区：
文献类型：
--
作者：
Ito, T;Tajima, F;Ogawa, M
目标。有报道称，胎鼠造血干细胞为CD34(+)，而成体干细胞为CD34(-)。我们试图描绘出造血干细胞CD34表达的发育变化，并对围产期、幼年和成年小鼠骨髓和/或血液中长期移植细胞进行了系统分析。为了获得干细胞总数的信息，我们制备了没有事先浓缩的单个核细胞CD34(+)和CD34(-)群体，并通过移植到致死照射的Ly-5同源小鼠来检测它们的长期重建能力。结果从围生期到5周龄的小鼠所有干细胞都是CD34(+)。在7周龄的小鼠中，CD3(4)-干细胞开始出现，在10周龄和IO周龄的小鼠中，大多数干细胞仅表达CD34(-)，约20%的成年干细胞表达CD34。大多数小鼠干细胞的CD34表达从阳性到阴性的发育变化发生在7-10周龄。大约20%的成体干细胞仍然是CD34(+)。这些观察结果为当前关于成人造血干细胞表达CD34的争议提供了洞察力，并表明人脐血和幼儿骨髓中的大多数干细胞是CD34(+)。(C)2000年国际实验血液学学会。爱思唯尔科学公司出版。
Objective. It has been reported that fetal murine hematopoietic stem cells are CD34(+), whereas adult stem cells are CD34(-). We sought to delineate the developmental changes of CD34 expression by hematopoietic stem cells and carried out systematic analysis of long-term engrafting cells in the bone marrow and/or blood of perinatal, juvenile, and adult mice.Materials and Methods. To obtain information on the total population of stem cells, we prepared CD34(+) and CD34(-) populations of mononuclear cells without prior enrichment and assayed their long-term reconstituting abilities by transplantation into lethally irradiated Ly-5 congenic mice.Results, All stem cells from perinatal to 5-week-old mice were CD34(+). In 7-week-old mice, CD3(4)- stem cells began to emerge, and the majority of the stem cells mere CD34(-) in the 10- and IO-week-old mice, Approximately 20% of adult stem cells expressed CD34.Conclusions. Developmental changes of CD34 expression from the positive to the negative state takes place between 7 and 10 weeks of age for the majority of murine stem cells. Approximately 20% of adult stem cells remain CD34(+). These observations provide insight into the current controversy regarding CD34 expression by adult hematopoietic stem cells and suggest that the majority of stem cells in human umbilical cord blood and bone marrow of young children are CD34(+). (C) 2000 International Society for Experimental Hematology. Published by Elsevier Science Inc.