STIMULATION OF INTRA-UTERINE GROWTH IN RAT BY α-MELANOCYTE-STIMULATING HORMONE
STIMULATION OF INTRA-UTERINE GROWTH IN RAT BY α-MELANOCYTE-STIMULATING HORMONE
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α-黑素细胞刺激激素对大鼠子宫内生长的刺激
DOI：
10.1677/joe.0.0700445
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发表时间：
1976
影响因子：
4
通讯作者：
F. Tilders
中科院分区：
文献类型：
--
作者：
D. Swaab;M. Visser;F. Tilders
：我们研究了内源性 α-MSH 是否具有刺激大鼠子宫内生长的功能。使用的方法是确定在子宫内生长突增期间是否存在这种激素，以及抗体与内源性胎儿 α-MSH 的结合是否会抑制这种生长。针对 α-MSH 或 ACTH 1-24 的抗体，无论是纯化的还是未纯化的，都会在成年雄性对照大鼠的中叶中诱导免疫荧光。使用纯化的抗 α-MSH，在怀孕第 18 天，即首次发现具有生物活性的 MSH 的那天，胎儿中间叶中出现了荧光。仅在妊娠第19天观察到垂体MSH含量与胎儿体重呈负相关。注射纯化的抗-α-MSH 会导致胎儿体重下降，但没有观察到对胎盘重量的影响。纯化的抗Acth 1-24对体重没有影响，但导致胎盘重量增加。这些结果支持了我们之前的发现，并表明内源性 MSH 具有刺激胎儿生长的功能。
: We have studied whether endogenous alpha-MSH has a function in stimulating intra-uterine growth in the rat. The approach used was to determine whether or not this hormone is present during the intra-uterine growth spurt, and if binding of endogenous foetal alpha-MSH by antibodies would inhibit this growth. Antibodies against alpha-MSH or ACTH 1-24, either purified or non-purified, induced immunofluorescence in the intermediate lobe of adult male control rats. Using purified anti-alpha-MSH, fluorescence appeared in the foetal intermediate lobe on day 18 of pregnancy, the day that biologically active MSH was first seen. A negative correlation was observed between the pituitary MSH content and foetal body weight only on day 19 of pregnancy. Injection of purified anti-alpha-MSH induced a drop in foetal body weight, but no effect on placental weight was observed. Purified anti-acth 1-24 had no effect upon body weight but caused an increase in placental weight. These results support our previous findings and indicate that endogenous MSH has a function in the stimulation of foetal growth.