Age-dependent axonal expression of potassium channel proteins during development in mouse hippocampus
Age-dependent axonal expression of potassium channel proteins during development in mouse hippocampus
复制标题
小鼠海马发育过程中钾通道蛋白的年龄依赖性轴突表达
DOI：
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发表时间：
2010
影响因子：
2.3
通讯作者：
G. Ahnert
中科院分区：
文献类型：
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作者：
H. Prüss;G. Grosse;I. Brunk;R. Veh;G. Ahnert
海马网络的发育需要神经元活动，这是由各种钾通道的差异表达和分选形成的。海马神经元在成熟的同时，其离子通道也发生了明显的变化。离子通道发生的年龄依赖性维度是至关重要的，因为它与网络形成相互依赖地联系在一起。然而，关于出生后海马发育过程中钾通道的确切时间表达的数据很少。因此，我们研究了几个电压门控钾通道蛋白在海马体发育过程中在体内和原代培养物中的表达，专注于被分类到轴突室的通道。Kv1.1，Kv1.2，Kv1.4，和Kv3.4蛋白表现出相当大的时间变化的轴突定位神经元亚群之间。因此，海马神经元可能具有细胞类型特异性的通道区室化机制。因此，年龄依赖性钾通道蛋白的轴突分选提供了一种新的方法来区分海马神经元的功能类别，并可能扩展我们对海马电路和记忆处理的理解。
The development of the hippocampal network requires neuronal activity, which is shaped by the differential expression and sorting of a variety of potassium channels. Parallel to their maturation, hippocampal neurons undergo a distinct development of their ion channel profile. The age-dependent dimension of ion channel occurrence is of utmost importance as it is interdependently linked to network formation. However, data regarding the exact temporal expression of potassium channels during postnatal hippocampal development are scarce. We therefore studied the expression of several voltage-gated potassium channel proteins during hippocampal development in vivo and in primary cultures, focusing on channels that were sorted to the axonal compartment. The Kv1.1, Kv1.2, Kv1.4, and Kv3.4 proteins showed a considerable temporal variation of axonal localization among neuronal subpopulations. It is possible, therefore, that hippocampal neurons possess cell type-specific mechanisms for channel compartmentalization. Thus, age-dependent axonal sorting of the potassium channel proteins offers a new approach to functionally distinguish classes of hippocampal neurons and may extend our understanding of hippocampal circuitry and memory processing.