Detection and Characterization of Novel Rotavirus Strains in the United States
Detection and Characterization of Novel Rotavirus Strains in the United States
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美国新型轮状病毒株的检测和表征
DOI：
10.1128/jcm.37.7.2392-2392.1999
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发表时间：
1999
影响因子：
9.4
通讯作者：
R. Glass
中科院分区：
文献类型：
--
作者：
M. Ramachandran;J. Gentsch;U. Parashar;S. Jin;P. Woods;J. Holmes;C. Kirkwood;R. Bishop;H. Greenberg;S. Urasawa;G. Gerna;B. Coulson;K. Taniguchi;J. Bresee;R. Glass
我们最近在美国建立了一个轮状病毒毒株监测系统，以监测轮状病毒疫苗接种计划实施前后流行的G血清型，并确定不常见毒株的出现。在这项研究中，我们检查了1996年至1997年从美国10个城市的腹泻儿童中获得的348种轮状病毒株。采用单克隆抗体免疫分析、逆转录- pcr和杂交相结合的方法对菌株进行了P型和G型、亚群和电泳型的鉴定。世界范围内最常见的4种菌株占分离株的83% (P[8]G1占66.4%；P[4]G2占8.3%；P[8]G3占6.9%；P[8]G4占1.4%)，但9.2%是罕见菌株（P[6]G9占5.5%；P[8]G9占1.7%；P[6]G1占1.4%；P[4]G1和P[8]G2各占0.3%）。不属于P型和G型的菌株占总数的5.5%，而具有一种以上G型（混合感染）的菌株占2.3%。10个城市中有4个城市检测到P[6]G9株具有短型和I亚群特异性，而P[8]G9株具有长型和II亚群VP6抗原。VP7开放阅读框的序列分析（与G9原型菌株WI61的VP7基因约有94 ~ 95%的氨基酸一致性）和与G9特异性单克隆抗体的结合均强烈提示美国G9菌株属于G9血清型。在美国多个城市，具有G9（7.2%）或P[6]（6.9%）特异性的异常轮状病毒的高检出率提示出现了新的毒株或过去的诊断不充分。这些毒株的流行病学重要性仍有待确定。
We recently established a rotavirus strain surveillance system in the United States to monitor the prevalent G serotypes before and after the anticipated implementation of a vaccination program against rotavirus and to identify the emergence of uncommon strains. In this study, we examined 348 rotavirus strains obtained in 1996 to 1997 from children with diarrhea in 10 U.S. cities. Strains were characterized for P and G types, subgroups, and electropherotypes by using a combination of monoclonal antibody immunoassay, reverse transcription-PCR, and hybridization. The four strains most commonly found worldwide comprised 83% of the isolates (P[8]G1, 66.4%; P[4]G2, 8.3%; P[8]G3, 6.9%; P[8]G4, 1.4%), but 9.2% were unusual strains (P[6]G9, 5.5%; P[8]G9, 1.7%; P[6]G1, 1.4%; and P[4]G1 and P[8]G2, 0.3% each). Strains not typeable for P or G type accounted for 5.5% of the total, while 2.3% of the strains had more than one G type (mixed infections). All P[6]G9 strains tested had short electropherotypes and subgroup I specificity and were detected in 4 of 10 cities, while P[8]G9 strains had long electropherotypes and subgroup II VP6 antigens. Both sequence analysis of the VP7 open reading frame (about 94 to 95% amino acid identity with the VP7 gene of G9 prototype strain WI61) and binding to a G9-specific monoclonal antibody strongly suggest that U.S. G9 strains belong to serotype G9. The high detection rates of unusual rotaviruses with G9 (7.2%) or P[6] (6.9%) specificity in multiple U.S. cities suggest the emergence of new strains or inadequate diagnosis in the past. The epidemiologic importance of these strains remains to be determined.