Dextran Sulfate Stimulated Fibrinolytic Activity In Whole Human Plasma: Dependence On The Contact Activation System And On 4 Urokinase-Related Antigen
Dextran Sulfate Stimulated Fibrinolytic Activity In Whole Human Plasma: Dependence On The Contact Activation System And On 4 Urokinase-Related Antigen
复制标题
硫酸葡聚糖刺激全人血浆中的纤溶活性：依赖于接触激活系统和 4 种尿激酶相关抗原
DOI：
10.1055/s-0038-1652619
复制
发表时间：
1981
影响因子：
6.7
通讯作者：
J. Griffin
中科院分区：
文献类型：
--
作者：
L. Miles;Z. Rothschild;J. Griffin
研究了在硫酸葡聚糖存在下全人血浆中纤维蛋白溶解活性的产生。将血浆与 N-氟芬酰胺-β-丙氨酸预孵育，以去除其抗纤溶酶和抗激活剂活性，然后与 50 μg/ml 硫酸葡聚糖 (Mr - 500,000) 在 40 ℃ 孵育 30 分钟。在 125I-纤维蛋白板上评估，30 分钟内的初始纤溶活性相当于约 9 ng/ml 纯化的纤溶酶。一部分针对纤溶酶原的山羊抗体阻断了受刺激血浆的纤维蛋白溶解活性，表明该活性是纤溶酶原依赖性的。前激肽释放酶或因子 XII（哈格曼因子）遗传缺陷的血浆显示出响应硫酸葡聚糖的纤溶活性初始速率降低。然而，在刺激的缺陷血浆与纤维蛋白长时间孵育（〜3小时）后，纤维蛋白溶解活性接近刺激的正常血浆。当正常血浆与针对纯化尿激酶的山羊抗体的γ级分预孵育时，硫酸葡聚糖刺激的纤溶活性以剂量依赖性方式显着降低。数据表明，在硫酸葡聚糖和N-氟芬酰胺-β-丙氨酸存在下，在全人血浆中刺激的纤溶活性取决于接触激活系统的蛋白质以及与尿激酶免疫学相关的分子。
The generation of fibrinolytic activity in whole human plasma in the presence of dextran sulfate was studied. Plasma was preincubated with N-flufenamyl-β-alanine to remove its antiplasmin and anti activator activities and then incubated with 50 μg/ml dextran sulfate (Mr - 500,000) for 30 min at 40. The initial fibrinolytic activity in 30 min, as assessed on a 125I-fibrin plate, was equivalent to approximately 9 ng/ml purified plasmin. A fraction of goat antibodies to plasminogen blocked the fibrinolytic activity of stimulated plasma, indicating that the activity was plasminogen dependent. Plasmas genetically deficient in either prekallikrein or Factor XII (Hageman Factor) showed a diminished initial rate of generation of fibrinolytic activity in response to dextran sulfate. However, after prolonged incubation (∼3 hr) of stimulated deficient plasmas with fibrin, the fibrinolytic activity approached that of stimulated normal plasma. When normal plasma was preincubated with the gamma fraction of goat antibodies made against purified urokinase, the dextran sulfate stimulated fibrinolytic activity was markedly decreased in a dose dependent manner. The data suggest that the fibrinolytic activity stimulated in whole human plasma in the presence of dextran sulfate and N-flufenamyl-β-alanine is dependent upon proteins of the contact activation system and also upon molecules immunologically related to urokinase.