REGULATORY ELEMENTS THAT MODULATE EXPRESSION OF HUMAN C-MYC
REGULATORY ELEMENTS THAT MODULATE EXPRESSION OF HUMAN C-MYC
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DOI：
10.1101/gad.1.7.659
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发表时间：
1987-09-01
影响因子：
10.5
通讯作者：
LEVENS, D
中科院分区：
文献类型：
--
作者：
HAY, N;BISHOP, JM;LEVENS, D
原癌基因c-myc的转录调控显然在细胞增殖和多种肿瘤的发生中起着重要作用。在这里，我们报告了人类c-myc的两个主要启动子（P1和P2）的转录本丰度是由位于基因上游2.3 kb结构域的正调控因子和负调控因子的组合控制的。在活跃增殖的细胞中，阳性因子的作用明显优于我们已经确定的单一负调节因子的作用。核蛋白与负调节因子和至少一种正调节因子内的核苷酸序列特异性结合。这里描述的调控元件的合作和对抗作用可能有助于c-myc转录的可塑性，并可能受到有时困扰c-myc的致瘤性损伤的影响。
Regulation of transcription from the proto-oncogene c-myc apparently plays an important part in cellular proliferation and the genesis of diverse tumors. Here, we report that the abundance of transcripts from the two principal promoters for human c-myc (P1 and P2) is governed by a composite of positive and negative regulators, located within a 2.3-kb domain upstream of the gene. In actively proliferating cells, the action of the positive elements is apparently dominant over that of the single negative regulator that we have identified. Nuclear proteins bind specifically to nucleotide sequences within the negative regulator and at least one of the positive regulators. The cooperative and counteracting actions of the regulatory elements described here presumably contribute to the plasticity of transcription from c-myc and may be affected by the tumorigenic damage that sometimes afflicts c-myc.