The paracrine effect of adipose-derived stem cells inhibits osteoarthritis progression.
The paracrine effect of adipose-derived stem cells inhibits osteoarthritis progression.
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DOI：
10.1186/s12891-015-0701-4
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发表时间：
2015-09-03
影响因子：
2.3
通讯作者：
Tsuchiya H
中科院分区：
文献类型：
--
作者：
Kuroda K;Kabata T;Hayashi K;Maeda T;Kajino Y;Iwai S;Fujita K;Hasegawa K;Inoue D;Sugimoto N;Tsuchiya H
本研究旨在确定关节内注射脂肪干细胞（ADSCs）是否抑制兔前交叉韧带横断（ACLT）模型中骨关节炎（OA）发展过程中的关节软骨退变。采用共培养系统研究脂肪干细胞对软骨细胞的旁分泌作用。对12只家兔的双膝关节进行ACLT。从皮下脂肪组织中分离ADSC。将含有透明质酸的ADSCs关节内注射到左膝，并将透明质酸注射到右膝。在8周和12周时，对膝关节进行肉眼、组织学和免疫化学比较。此外，使用ADSCs和软骨细胞的共培养系统测定细胞活力。从宏观上看，ACLT后8周，ADSC治疗膝关节的骨关节炎进展比对照膝关节轻。在组织学上，对照组膝关节在8周时内侧和外侧髁均显示出明显的侵蚀，而软骨主要保留在ADSC治疗的膝关节中。在12周时，ADSC治疗的膝关节显示出对软骨退化的轻微抑制，与对照膝关节不同。免疫组化，MMP-13的表达在ADSC治疗的软骨比对照膝关节。与ADSCs共培养的软骨细胞的细胞活力高于单独培养的软骨细胞。TNF-α诱导的凋亡刺激在两组之间相似。关节内注射的ADSC通过归巢到滑膜并分泌具有软骨保护作用（如软骨细胞增殖和软骨基质保护）的液体因子来抑制软骨变性进展。
This study aimed to determine whether intra-articularly injected adipose-derived stem cells (ADSCs) inhibited articular cartilage degeneration during osteoarthritis (OA) development in a rabbit anterior cruciate ligament transection (ACLT) model. The paracrine effects of ADSCs on chondrocytes were investigated using a co-culture system. ACLT was performed on both knee joints of 12 rabbits. ADSCs were isolated from the subcutaneous adipose tissue. ADSCs with hyaluronic acid were intra-articularly injected into the left knee, and hyaluronic acid was injected into the right knee. The knees were compared macroscopically, histologically, and immunohistochemically at 8 and 12 weeks. In addition, cell viability was determined using co-culture system of ADSCs and chondrocytes. Macroscopically, osteoarthritis progression was milder in the ADSC-treated knees than in the control knees 8 weeks after ACLT. Histologically, control knees showed obvious erosions in both the medial and lateral condyles at 8 weeks, while cartilage was predominantly retained in the ADSC-treated knees. At 12 weeks, the ADSC-treated knees showed a slight suppression of cartilage degeneration, unlike the control knees. Immunohistochemically, MMP-13 expression was less in the ADSC-treated cartilage than in the control knees. The cell viability of chondrocytes co-cultured with ADSCs was higher than that of chondrocytes cultured alone. TNF-alpha-induced apoptotic stimulation was similar between the two groups. Intra-articularly injected ADSCs inhibited cartilage degeneration progression by homing to the synovium and secreting a liquid factor having chondro-protective effects such as chondrocyte proliferation and cartilage matrix protection.