Molecular characterization of the expression of distinct classes of cyclins during the early development of tomato fruit
Molecular characterization of the expression of distinct classes of cyclins during the early development of tomato fruit
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DOI：
10.1007/s004250000306
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发表时间：
2000-08-01
期刊：
影响因子：
4.3
通讯作者：
Chevalier, C
中科院分区：
文献类型：
--
作者：
Joubès, J;Walsh, D;Chevalier, C
番茄果实早期发育的研究从两个不同的生长阶段，基本上是细胞分裂和细胞扩增进行。在这项研究中，我们调查的关键细胞周期调控，有丝分裂和G1期细胞周期蛋白，在番茄果实发育过程中的表达特征。我们分离了六个基因命名为Lyces：CycA 1;1，Lyces; CycA 2;1，Lyces; CycA 3：1，Lyces; CycB 1;1和Lyces; CycB 2;1编码番茄有丝分裂细胞周期蛋白，和Lyces; CycD 3;1编码G1细胞周期蛋白。转录本的积累主要与有丝分裂活跃的器官有关：发育中的果实、嫩叶和根，以及在适当的糖补料条件下的细胞悬浮培养物。所有分离的细胞周期蛋白基因的转录本可以检测到在表皮和果皮的果实组织中仍然存在一些轻微的有丝分裂活动在开始成熟。然而，吕塞斯; CycA3; 1和Lyces; CycD 3：1在果实发育后期的凝胶组织中表达，表明它们参与了凝胶组织中分化细胞和巨细胞的核内复制。
Early fruit development in tomato (Lycopersicon esculentum Mill.) proceeds from two distinct phases of growth, essentially cell division and cell expansion. In this study, we investigated the expression characteristics of the key cell-cycle regulators, mitotic and G1 cyclins, during tomato fruit development. We isolated six genes designated Lyces: CycA1;1, Lyces;CycA2;1, Lyces; CycA3:1, Lyces;CycB1;1 and Lyces;CycB2;1 encoding tomato mitotic cyclins, and Lyces;CycD3;1 encoding a G1 cyclin. The accumulation of transcripts was predominantly associated with mitotically active organs: developing fruits, young leaves and roots, and with cell-suspension cultures under appropriate sugar feeding conditions. Transcripts for all the isolated cyclin genes could be detected in the epidermis and pericarp of fruit tissues where some slight mitotic activity still remained at the onset of ripening. However, Lyces;CycA3;1 and Lyces;CycD3:1 were expressed in the gel tissue at the late stage of fruit development, suggesting that they are involved in endoreduplication of the differentiated and giant cells of the gel tissue.