Alterations in mRNA expression of BACE1, cathepsin B, and glutaminyl cyclase in mice ischemic brain
Alterations in mRNA expression of BACE1, cathepsin B, and glutaminyl cyclase in mice ischemic brain
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DOI：
10.1097/wnr.0b013e328332024a
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发表时间：
2009-10-28
期刊：
影响因子：
1.7
通讯作者：
Liu, Pei-qing
中科院分区：
文献类型：
--
作者：
Ye, Jian-tao;Pi, Rong-biao;Liu, Pei-qing
脑缺血和阿尔茨海默病之间的关系已被广泛评估。然而，脑缺血与P-淀粉样蛋白（AP）沉积之间的关系仍有待澄清。在此，我们使用小鼠双侧颈总动脉结扎模型来研究短暂性全脑缺血后AP前体蛋白裂解酶1（BACE 1）、组织蛋白酶B和γ-氨基环化酶mRNA表达的变化。逆转录聚合酶链反应（RT-PCR）结果显示，这3个A β代谢相关基因在脑组织中的表达均以不同方式上调。提示这3个A β代谢相关基因均参与了短暂性脑缺血后急性和慢性AP的发生，为进一步了解脑缺血与Alzheimer病的发病机制奠定了基础。NeuroReport 20：1456-1460（C）2009 Wolters Kluwer Health竖条Lippincott威廉姆斯& Wilkins.
The relationship between cerebral ischemia and Alzheimer's disease has been evaluated extensively. However, the association between cerebral ischemia and the deposition of P-amyloid (AP) remains to be clarified. Here, we used mice bilateral common carotid artery ligation model to investigate the alterations in mRNA expression of AP precursor protein cleavage enzyme 1(BACE1), cathepsin B, and glutaminyl cyclase after transient global cerebral ischemia. The reverse-transcriptase PCR assay showed that the expressions of these three A beta-metabolism-related genes were upregulated in brain with different manner. It indicates that all these three A beta-metabolism-related genes may participate in the acute and chronic AP generation after transient cerebral ischemia, and will be helpful to understand the mechanisms underlying the linkage of brain ischemia and Alzheimer's disease. NeuroReport 20:1456-1460 (C) 2009 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.