Label-Free and Multiplex Detection of Antibiotic Residues in Milk Using Imaging Surface Plasmon Resonance-Based Immunosensor
Label-Free and Multiplex Detection of Antibiotic Residues in Milk Using Imaging Surface Plasmon Resonance-Based Immunosensor
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DOI：
10.1021/ac901230v
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发表时间：
2009-09-15
影响因子：
7.4
通讯作者：
Norde, Willem
中科院分区：
文献类型：
--
作者：
Raz, Sabina Rebe;Bremer, Maria G. E. G.;Norde, Willem
对食品中抗菌药物残留的监测在很大程度上依赖于获得足够的分析技术。目前，需要一种具有广谱检测范围的高通量筛选方法。本文介绍了一种基于成像表面等离子体共振（iSPR）平台的微阵列生物传感器的开发，用于定量和同时免疫检测牛奶中不同抗生素残留。采用单传感器芯片检测四个主要抗生素家族的模型化合物：氨基糖苷类（新霉素、庆大霉素、卡那霉素和链霉素）、磺胺类（磺胺乙嗪）、苯尼醇类（氯霉素）和氟喹诺酮类（恩诺沙星）。通过在竞争性格式中复用7种免疫测定，我们能够在缓冲液和10倍稀释的牛奶中以十亿分之一（ppb）的水平测量所有目标化合物。对新霉素、卡那霉素、链霉素、恩诺沙星和磺胺乙嗪的检测足够灵敏，可用于欧盟规定的牛奶最大残留水平的控制。在检测灵敏度和鲁棒性方面，该生物传感器的总体性能与传统的四通道表面等离子体共振（SPR）生物传感器相当。结合SPR传感器和微阵列的优点，这里描述的生物传感器的利用为现有方法提供了一个有希望的替代方案，并且与多分析物食品分析高度相关。
Monitoring of antimicrobial drug residues in foods relies greatly on the availability of adequate analytical techniques. Currently, there is a need for a high-throughput screening method with a broad-spectrum detection range. This paper describes the development of a microarray biosensor, based on an imaging surface plasmon resonance (iSPR) platform, for quantitative and simultaneous immunodetection of different antibiotic residues in milk. Model compounds from four major antibiotic families: aminoglycosides (Neomycin, Gentamicin, Kanamycin, and Streptomycin), sulfonamides (Sulfamethazine), fenicols (Chloramphenicol), and fluoroquinolones (Enrofloxacin) were detected using a single sensor chip. By multiplexing seven immunoassays in a competitive format, we were able to measure all the target compounds at parts per billion (ppb) levels in buffer and in 10x-diluted milk. The assays for Neomycin, Kanamycin, Streptomycin, Enrofloxacin, and Sulfamethazine were sensitive enough for milk control at maximum residue levels as established in the European Union. The overall performance of the biosensor was determined to be comparable to that of conventional four-channel surface plasmon resonance (SPR)-based biosensors, in terms of assay sensitivity and robustness. Combining the advantages of a SPR sensor and a microarray, utilization of the biosensor described here offers a promising alternative to the existing methods and is highly relevant for multianalyte food profiling.