Decreased level of vascular endothelial growth factor in bronchoalveolar lavage fluid of normal smokers and patients with pulmonary fibrosis
Decreased level of vascular endothelial growth factor in bronchoalveolar lavage fluid of normal smokers and patients with pulmonary fibrosis
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DOI：
10.1164/rccm.2103112
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发表时间：
2002-08-01
影响因子：
24.7
通讯作者：
Izumi, T
中科院分区：
文献类型：
--
作者：
Koyama, S;Sato, E;Izumi, T
血管内皮生长因子（VEGF）在血管的发育和血管功能的维持中起着多功能的作用。VEGF的减少减少血管生成并诱导血管内皮细胞的凋亡。VEGF受体的抑制导致内皮细胞凋亡和肺气肿。我们推测，VEGF浓度可能会降低慢性肺部疾病患者。采用酶联免疫吸附法检测正常吸烟者、非吸烟志愿者、特发性肺纤维化、结缔组织病相关性肺纤维化和结节病患者支气管肺泡灌洗液（BALF）中VEGF水平。采用高效液相色谱法测定BALF中VECF的亚型。非吸烟志愿者中的VECF在高浓度下可检测到。相反，大多数正常吸烟者的VEGF低于检测限。在非吸烟志愿者BALF中发现的VEGF为VEGF 165。与不吸烟志愿者相比，特发性肺纤维化、结缔组织病相关性肺纤维化和结节病患者的VECF显著降低。吸烟组VEGF表达进一步降低。这些数据表明，吸烟者和慢性肺部疾病患者中VEGF的降低可能减少血管生成并诱导血管内皮细胞凋亡。
Vascular endothelial growth factor (VEGF) plays multifunctional roles in both the development of vasculature and the maintenance of vascular function. A decrease in VEGF reduces angiogenesis and induces apoptosis of vascular endothelial cells. Inhibition of the VEGF receptor causes endothelial cell apoptosis and emphysema. We postulated that VEGF concentrations might be reduced in patients with chronic lung diseases. The level of VEGF was evaluated by enzyme-liked immunosorbant assay in bronchoalveolar lavage fluid (BALF) from normal smokers, nonsmoking volunteers, idiopathic pulmonary fibrosis, pulmonary fibrosis associated with a connective tissue disease, and sarcoidosis. The isoforms of VECF in BALF were determined by high-performance liquid chromatography. VECF in nonsmoking volunteers was detectable at a high concentration. In contrast, VEGF in most of the normal smokers was below the detectable limit. The VEGF found in nonsmoking volunteers BALF was VEGF165. VECF was significantly decreased in idiopathic pulmonary fibrosis, pulmonary fibrosis associated with a connective tissue disease, and sarcoidosis compared with nonsmoking volunteers. The smoking patients showed a further decrease in VEGF. These data suggest that the decrease in VEGF in smokers and patients with chronic lung diseases may reduce angiogenesis and induce apoptosis of vascular endothelial cells.