Metabotropic glutamate receptors inhibit GABA release in rat histamine neurons
Metabotropic glutamate receptors inhibit GABA release in rat histamine neurons
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DOI：
10.1016/j.neulet.2014.07.022
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发表时间：
2014-09-05
影响因子：
2.5
通讯作者：
Jang, Il-Sung
中科院分区：
文献类型：
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作者：
Jang, Il-Sung
使用传统的全细胞膜片钳技术在组胺能神经元中检查代谢型谷氨酸 (mGIu) 受体对 GABA 能传递的调节。 DHPG 是一种选择性 I 族 mGlu 受体激动剂，对 GABA 能抑制性突触后电流 (IPSC) 没有影响。然而，选择性II族或III族mGlu受体激动剂DCG-IV (1 μM)和L-AP4 (1 μM)分别降低了GABA能IPSC的振幅，同时增加了配对脉冲比。 DCG-IV的抑制作用被200nM LY341495（II组和III组mGlu受体拮抗剂）完全阻断，L-AP4的抑制作用被10μM LY341495完全阻断。这些结果表明多种 mGlu 受体参与调节组胺能神经元的兴奋性。 (C) 2014 Elsevier Ireland Ltd. 保留所有权利。
The modulation of GABAergic transmission by metabotropic glutamate (mGIu) receptors was examined in histaminergic neurons using a conventional whole-cell patch clamp technique. DHPG, a selective group I mGlu receptor agonist, had no effect on GABAergic inhibitory postsynaptic currents (IPSCs). However, DCG-IV (1 mu M) and L-AP4 (1 mu M), selective group II or III mGlu receptor agonists, respectively, decreased the amplitude of GABAergic IPSCs and simultaneously increased the paired-pulse ratio. The inhibitory effect of DCG-IV was completely blocked by 200 nM LY341495, a group II and III mGlu receptor antagonist, and the inhibitory effect of L-AP4 was completely blocked by 10 mu M LY341495. These results suggest that multiple mGlu receptors are involved in regulating the excitability of histaminergic neurons. (C) 2014 Elsevier Ireland Ltd. All rights reserved.