DNA–inorganic hybrid material as selective absorbent for harmful compounds
DNA–inorganic hybrid material as selective absorbent for harmful compounds
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DOI：
10.1016/j.polymer.2008.08.027
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发表时间：
2008-10
期刊：
影响因子：
4.6
通讯作者：
M. Yamada;Hirofumi Aono
中科院分区：
文献类型：
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作者：
M. Yamada;Hirofumi Aono
双链DNA是一种功能性高分子材料，但大量的DNA来源，如鲑鱼鱼精和贝类性腺，已被作为工业废料丢弃。因此，将这些废弃的DNA转化为有用的材料将有利于利用DNA的独特性质。包括DNA在内的这些材料已经通过与有机聚合物如海藻酸、胶原和壳聚糖混合来制备。然而，由于这些材料完全由有机成分组成，因此这些材料不具有材料的机械强度。因此，我们将DNA与硅烷偶联剂双（三甲氧基硅基丙基）胺或双[（3-三甲氧基硅基）丙基]乙二胺混合，制备了有机-无机杂化材料。这些具有柔性的杂化材料不溶于水，并且耐核酸酶水解。此外，这种杂化材料的机械强度约为未与硅烷偶联剂混合的DNA的两倍。此外，杂交材料中的双链DNA在水溶液中保持了B型结构。因此，我们证明了DNA作为功能材料的利用。因此，这种材料可以选择性地积累具有平面结构的有害DNA嵌入化合物，如二苯并对二恶英，二苯并呋喃和溴化乙锭。含双链DNA的有机-无机杂化材料具有作为生物材料应用于医学、工程和环境等领域的潜力。
Double-stranded DNA is one of functional polymers, but the large amounts of DNA sources, such as salmon milt and shellfish gonads, have been discarded as industrial wastes. Therefore, conversion of this discarded DNA to be a useful material would be beneficial to utilize the unique property of DNA. These materials including DNA have been prepared by mixing with the organic polymers, such as alginic acid, collagen, and chitosan. However, since these materials have consisted from entirely organic components, these do not have the mechanical strength for a material. So, we prepared the organic–inorganic hybrid materials by mixing DNA with silane coupling reagents bis(trimethoxysilylpropyl)amine or bis[(3-trimethoxysilyl)propyl]ethylenediamine. These hybrid materials with the flexibility were water-insoluble and resistant to hydrolysis by nuclease. In addition, the mechanical strength of this hybrid material was approximately twice as high as that of DNA without mixing with silane coupling reagents. Furthermore, the double-stranded DNA in the hybrid materials has been maintained in a B-form structure in aqueous solution. Thus, we demonstrated the utilization of DNA as a functional material. As a result, this material could selectively accumulate harmful DNA-intercalating compounds with the planar structure, such as dibenzo-p-dioxin, dibenzofuran, and ethidium bromide. Organic–inorganic hybrid material including double-stranded DNA has potential to serve as a useful biomaterial for medical, engineering, and environmental applications.