Antbiotic-resistant Salmonella and Escherichia coli isolates with integrons and extended-spectrum beta-lactamases in surface water and sympatric black-headed gulls
Antbiotic-resistant Salmonella and Escherichia coli isolates with integrons and extended-spectrum beta-lactamases in surface water and sympatric black-headed gulls
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DOI：
10.1111/j.1365-2672.2009.04155.x
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发表时间：
2009-06-01
影响因子：
4
通讯作者：
Cizek, A.
中科院分区：
文献类型：
--
作者：
Dolejska, M.;Bierosova, B.;Cizek, A.
目的：对捷克东北部某池塘地表水和同一池塘黑头鸥幼雏筑巢水中的耐抗生素沙门氏菌和大肠杆菌进行检测。方法与结果：水样品中沙门氏菌检出率为16% (n = 87)，海鸥样品中检出率为24% （n = 216）。肠炎沙门氏菌PT8和PT4最常见。在12% （n = 14）的水和28% （n = 51）的海鸥沙门氏菌中发现了抗生素耐药性。池水样本检出大肠杆菌83份（95%），海鸥样本检出大肠杆菌213份（99%）。共有18% （n = 83）的水和28% （n = 213）的海鸥大肠杆菌分离株对抗菌药物产生耐药性。在21% （n = 14）的水和15% （n = 60）的海鸥耐药大肠杆菌分离株中发现了1类整合子。2类整合子和产生广谱β -内酰胺酶的大肠杆菌分离株（含bla（CTX-M-1）、bla（CTX-M-15）样、bla（SHV-2）和bla(SHV-12)）分别在13%（8株阳性，60株海鸥耐药大肠杆菌）和3%（7株阳性，216株海鸥耐药大肠杆菌）中检测到。具有相同脉冲场凝胶电泳（PFGE）模式的耐药大肠杆菌分离株在海鸥或水中均有发现，但两者均未发现。在海鸥和水中均发现了相同血清型和PFGE谱的沙门氏菌。结论：池塘水体和同域黑头鸥中均存在较高的耐药沙门氏菌和大肠杆菌感染率。研究的意义和影响：记录了耐抗生素大肠杆菌和沙门氏菌对池塘地表水的严重污染。黑头鸥被确定为耐抗生素沙门氏菌和大肠杆菌的重要宿主，包括广谱β -内酰胺酶产生分离株。
Aim: To examine surface water from a pond in the northeastern part of the Czech Republic and young black-headed gulls (Larus ridibundus) nesting oil the same pond for the presence of antibiotic-resistant Salmonella and Escherichia coli.Methods and Results: A total of 16% (n = 87) of water and 24% (n = 216) of gull samples yielded Salmonella. Salmonella Enteritidis PT8 and PT4 were the most prevalent. Antibiotic resistance was found in 12% (n = 14) of water and 28% (n = 51) Of gull salmonellae. Escherichia coli were found in 83 (95%) and 213 (99%) of pond water and gull samples, respectively. Totals of 18% (n = 83) of water and 28% (n = 213) of gull E. coli isolates were resistant to antimicrobial agents tested. Class 1 integrons were found in 21% (n = 14) of water and 15% (n = 60) of gull antibiotic-resistant E. coli isolates. Class 2 integrons and extended-spectrum beta-lactamase-producing E. coli isolates (with bla(CTX-M-1), bla(CTX-M-15)-like, bla(SHV-2) and bla(SHV-12)) were found in 13% (eight positive, n = 60 gull-resistant E. coli isolates) and 3% (seven positive, n = 216 gull E. coli isolates) of gull isolates, respectively. Antibiotic-resistant E. coli isolates with identical Pulsed field gel electrophoresis (PFGE) patterns were found in either gulls or water, but not both. Salmonellae of the same serotype and PFGE profile were found in both gulls and water.Conclusion: A high prevalence of antibiotic-resistant salmonellae and E. coli were found in both pond water and in sympatric black-headed gulls.Significance and Impact of the Study: Intensive contamination of pond surface water by antibiotic-resistant E. coli and salmonellae was documented. Black-headed gulls were identified as important reservoirs of antibiotic-resistant salmonellae and E. coli, including extended-spectrum beta-lactamase-producing isolates.