Tensile Force Transmission in Human Patellar Tendon Fascicles Is Not Mediated by Glycosaminoglycans
Tensile Force Transmission in Human Patellar Tendon Fascicles Is Not Mediated by Glycosaminoglycans
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DOI：
10.3109/03008207.2010.551569
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发表时间：
2011-10-01
影响因子：
2.9
通讯作者：
Magnusson, Stig P.
中科院分区：
文献类型：
--
作者：
Svensson, Rene B.;Hassenkam, Tue;Magnusson, Stig P.
肌腱的正确力学功能对人体生理至关重要，因此肌腱的力学性能已经成为研究的主题几十年了。然而，一个最基本的问题仍然没有答案：如何通过钢筋束传递载荷？有人建议，蛋白聚糖相关的糖胺聚糖（GAG）的表面上发现的胶原纤维可能是一个重要的传输器的负荷，但现有的结果是模糊的，并没有调查人类肌腱。我们已经使用了一个小规模的机械测试系统，以测量从人髌腱在两个不同的变形率之前和之后的GAGs去除软骨素酶ABC治疗的机械性能。使用二甲基亚甲基蓝测定来量化酶处理的效率。移除至少79%的GAG不会显著改变肌腱模量、相对能量耗散、峰值应力或峰值应变。变形速率的影响也不受处理的调制，表明对粘度没有影响。这些结果表明，GAG不能被认为是人类髌腱中张力传递的介质，因此，力传递必须通过其他基质组分发生，或者原纤维必须至少在7 mm的测试长度上是机械连续的。
Correct mechanical function of tendons is essential to human physiology and therefore the mechanical properties of tendon have been a subject of research for many decades now. However, one of the most fundamental questions remains unanswered: How is load transmitted through the tendon? It has been suggested that the proteoglycan-associated glycosaminoglycans (GAGs) found on the surface of the collagen fibrils may be an important transmitter of load, but existing results are ambiguous and have not investigated human tendons. We have used a small-scale mechanical testing system to measure the mechanical properties of fascicles from human patellar tendon at two different deformation rates before and after removal of GAGs by treatment with chondroitinase ABC. Efficiency of enzyme treatment was quantified using dimethylmethylene blue assay. Removal of at least 79% of the GAGs did not significantly change the tendon modulus, relative energy dissipation, peak stress, or peak strain. The effect of deformation rate was not modulated by the treatment either, indicating no effect on viscosity. These results suggest that GAGs cannot be considered mediators of tensile force transmission in the human patellar tendon, and as such, force transmission must either take place through other matrix components or the fibrils must be mechanically continuous at least to the tested length of 7 mm.