Formulation of curcumin delivery with functionalized single-walled carbon nanotubes: characteristics and anticancer effects in vitro
Formulation of curcumin delivery with functionalized single-walled carbon nanotubes: characteristics and anticancer effects in vitro
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DOI：
10.3109/10717544.2013.848246
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发表时间：
2014-08-01
期刊：
影响因子：
6
通讯作者：
Zhang, Zhenzhong
中科院分区：
文献类型：
--
作者：
Li, Haixia;Zhang, Nan;Zhang, Zhenzhong
背景：单壁碳纳米管是一类重要的人工纳米材料，具有独特的物理化学性质，被用作姜黄素的新型载体。目的：通过避开姜黄素在生理条件下极低的水溶性和不稳定性的传统限制，结合光热疗法，利用单壁碳纳米管在0.8-1.4 mm处的强烈光吸收导致局部过热，制备和评价姜黄素的抗癌作用。随后，对配方进行了尺寸、Zeta-电位和形貌分析。用荧光分光光度计测定了姜黄素的溶解度、稳定性和释放度，用X射线衍射法和紫外可见光谱测定了姜黄素的固体相。结果与讨论：在PC-3细胞中，SWCNT-CUR被功能化，随后的SWCNT-CUR结合物的平均尺寸为170.4 nm，Zeta电位为-12.5 mV，显著提高了姜黄素的溶解性和稳定性，克服了充分传递姜黄素的障碍。此外，姜黄素在SWCNT-CUR中以无定形形式存在，可以快速释放。在PC-3细胞中，与天然姜黄素相比，SWCNT-CUR具有更好的抑制效果。同时，SWCNT-Cur中的SWCNTs不仅可以作为支架，还可以作为热消融剂，进一步抑制PC-3细胞的生长。结论：SWCNT-Cur组装体可能为姜黄素在肿瘤治疗中的应用提供了一种有前景的递送系统。
Context: Single-walled carbon nanotubes (SWCNTs), an important class of artificial nanomaterials with unique physicochemical properties, were used as novel carriers of curcumin.Objective: Formulation and evaluation of curcumin-loaded SWCNTs systems for utilizing the curcumin's anticancer potential by circumventing conventional limitations of extremely low aqueous solubility and instability under physiological conditions, and combining SWCNTs photothermal therapy enabled by the strong optical absorbance of SWCNTs in the 0.8-1.4 mm resulting in excessive local heating.Methods: After functionalized SWCNTs were confirmed, they were conjugated with curcumin (SWCNT-Cur). Subsequently, the formulation was analyzed for size, zeta-potential and morphology. And the solubility, stability and release of curcumin were assessed using spectrofluorometer, and the solid state of the curcumin was determined using X-ray diffraction and UV spectroscopy. Furthermore, in PC-3 cells, photothermal response was further determined by irradiating laser after the antitumor effect of SWCNT-Cur was evaluated.Results and discussion: SWCNTs were functionalized, and subsequent SWCNT-Cur conjugates were found to possess an average size of 170.4 nm, a zeta potential of -12.5mV and to significantly enhance the solubility and stability of curcumin, overcoming the barriers to adequate curcumin delivery. Moreover, curcumin in SWCNT-Cur was in an amorphous form and could be rapidly released. In PC-3 cells, improved inhibition efficacy was achieved by SWCNT-Cur compared with native curcumin. Meanwhile, the SWCNTs in SWCNT-Cur served not only as scaffolds but also as thermal ablation agents, further inhibiting PC-3 cell growth.Conclusion: SWCNT-Cur assemblies may provide a promising delivery system for curcumin for use in cancer therapy.