A method for determining blood flow and oxygen consumption in the rat brain.
A method for determining blood flow and oxygen consumption in the rat brain.
复制标题
一种测定大鼠大脑血流量和耗氧量的方法。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
B. Siesjö
中科院分区：
文献类型：
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作者：
B. Nilsson;B. Siesjö
在氧化亚氮麻醉下，采用133氙改进的凯蒂和施密特惰性气体技术，从关节盂后静脉取脑静脉血，测定脑血流量（CBF）和脑氧代谢率（CMRo 2）。通过比较血液和组织中133氙活度降低的速率，研究了静脉血样本的脑外污染。在采样过程中，通过轻轻压迫对侧关节盂后静脉避免了污染。大鼠脑的CBF和CMRo_2分别为80 ± 2和7.6 ± 0.2ml ~（-1）·min ~（-1）。这些值比以前在类似条件下获得的大脑皮层组织的值低约25%。诱导的高碳酸血症（Paco 2约70 mm Hg）或低碳酸血症（Paco 2 15-20 mm Hg）引起CBF的预期变化，但未改变CMRo 2。大鼠大脑的CMRo 2至少是人类大脑的两倍。这种种属差异，这是类似于以前报道的体外脑组织的氧摄取，可能反映了脑重量和神经元堆积密度之间的反比关系。
Cerebral blood flow (CBF) and cerebral metabolic rate for oxygen (CMRo2) were measured in rats under nitrous oxide anaesthesia, using a 133Xenon modification of the Kety and Schmidt inert gas technique with sampling of cerebral venous blood from the retroglenoid vein. Extracerebral contamination of the venous blood sampled was studied by comparing the rates at which the activity of 133Xenon decreased in blood and tissues. Contamination was avoided by gentle compression of the contralateral retroglenoid vein during sampling. CBF and CMRo2 of the rat brain were 80+/-2 and 7.6+/-0.2 ml-(100g)-1-min-1, respectively. These values are about 25% lower than those previously obtained for cerebral cortical tissue under similar conditions. Induced hypercapnia (Paco2 about 70 mm Hg) or hypocapnia (Paco2 15-20 mm Hg) gave rise to expected changes in CBF but did not alter CMRo2. The CMRo2 of the rat brain is at least twice that of the human brain. This species difference, which is similar to that previously reported for the oxygen uptake of cerebral tissue in vitro, probably reflects on inverse relationship between brain weight and neuronal packing density.