Gene transfection into HeLa cells by vesicles containing cationic peptide lipid
Gene transfection into HeLa cells by vesicles containing cationic peptide lipid
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DOI：
10.1271/bbb.69.1453
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发表时间：
2005-08-01
影响因子：
1.6
通讯作者：
Kato, K
中科院分区：
文献类型：
--
作者：
Ohama, Y;Heike, Y;Kato, K
脂质囊泡作为药物和/或基因递送的微胶囊是潜在有用的。我们开发了主要由脱水山梨糖醇单油酸酯（Span 80）和阳离子肽脂质（CPL）组成的阳离子脂质囊泡，并评估CPL囊泡作为基因转染载体。发现用于基因转染到HeLa细胞中的最佳CPL浓度为总脂质的20重量%，并且这种CPL囊泡没有表现出显著的细胞毒性。在制备CPL囊泡-质粒复合物之前，多聚赖氨酸和质粒的共培养是有效的。使用LipofectAMINE的脂质转染在10%血清补充培养基中受到抑制。20重量% CPL囊泡的转染效率，但是，不受血清在培养基中的质粒用聚-L-赖氨酸处理时。
Lipid vesicles are potentially useful as microcapsules for drug and/or gene delivery. We developed cationic lipid vesicles consisting mainly of sorbitan monooleate (Span 80) and cationic peptide lipid (CPL), and evaluated the CPL vesicles as gene transfection vectors. The optimum CPL concentration for gene transfection into HeLa cells was found to be 20 wt % of total lipid, and such CPL vesicles did not exhibit significant cytotoxicity. Co-culture Of poly-L-lysine and plasmids prior to making CPL vesicle-plasmid complexes was effective. Lipofection using LipofectAMINE was suppressed in 10% serum-supplemented medium. The transfection efficiency of 20 wt % CPL vesicles, however, was not affected by serum in the medium when plasmids were treated with poly-L-lysine.