DIFFERENTIATION OF MOUSE MYELOID-LEUKEMIA CELLS INDUCED BY 1-ALPHA,25-DIHYDROXYVITAMIN-D3
DIFFERENTIATION OF MOUSE MYELOID-LEUKEMIA CELLS INDUCED BY 1-ALPHA,25-DIHYDROXYVITAMIN-D3
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DOI：
10.1073/pnas.78.8.4990
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
SUDA, T
中科院分区：
文献类型：
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作者：
ABE, E;MIYAURA, C;SUDA, T
小鼠髓性白血病细胞可在体外被1 α诱导分化为巨噬细胞，25-二羟维生素D3，维生素D3的活性形式。1 α的最小浓度，25-二羟维生素D3诱导细胞分化的浓度为0.12 nM。由12 nM 1 α诱导的各种标志物的细胞分化程度，25-二羟维生素D3几乎等同于由1 μ M地塞米松（已知最有效的刺激剂）诱导的。在通过1 α分化的几个标记中，25-二羟维生素D3，吞噬活性在24小时内诱导，随后诱导溶菌酶和运动活性。用0.01-1 μ M 1 α-β-葡聚糖诱导类似的变化。羟基维生素D3。25-羟基维生素D_3和24 R，25-二羟维生素D_3仅表现出弱的诱导活性。除了众所周知的增强肠道钙转运和骨矿物质动员的生物活性外，1 α，25-二羟维生素D3显然参与骨髓细胞的分化。
Mouse myeloid leukemia cells can be induced to differentiate into macrophages in vitro by 1.alpha.,25-dihydroxyvitamin D3, the active form of vitamin D3. The minimal concentration of 1.alpha.,25-dihydroxyvitamin D3 to induce the cell differentiation was 0.12 nM. The degree of cell differentiation in various markers induced by 12 nM 1.alpha.,25-dihydroxyvitamin D3 was nearly equivalent to that induced by 1 .mu.M dexamethasone, the most potent known stimulator. Among several markers of the differentiation by 1.alpha.,25-dihydroxyvitamin D3, phagocytic activity was induced within 24 h followed by induction of lysozyme and locomotive activities. Similar changes were induced by 0.01-1 .mu.M 1.alpha.-hydroxyvitamin D3. 25-Hydroxyvitamin D3 and 24R,25-dihydroxyvitamin D3 showed only weak inducing activity. In addition to its well-known biological activities in enhancing intestinal Ca transport and bone mineral mobilization, 1.alpha.,25-dihydroxyvitamin D3 is apparently involved in the differentiation of bone marrow cells.