Divergent postnatal development of the carotid body in DBA/2J and A/J strains of mice.
Divergent postnatal development of the carotid body in DBA/2J and A/J strains of mice.
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DBA/2J 和 A/J 品系小鼠颈动脉体的出生后发育存在差异。
DOI：
10.1152/japplphysiol.01477.2010
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
Shirahata,Machiko
中科院分区：
文献类型：
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作者：
Kostuk,EricW;Balbir,Alexander;Fujii,Koichi;Fujioka,Akiko;Pichard,LuisE;Shirahata,Machiko
我们以前已经表明，成年DBA/2 J和A/J品系小鼠颈动脉体体积和形态不同。问题是这些差异是在产前还是产后形成的。研究这些小鼠颈动脉体的形态发育和相关基因可以进一步了解颈动脉体的适当形成。我们检查了这些小鼠从1天到4周龄的颈动脉体的体积，形态和GDNF家族，Dlx 2，Msx 2和Phox 2b的基因表达的差异。这两个菌株表现出不同的形态开始在1周龄。DBA/2 J小鼠颈动脉体的体积从1周龄到2周龄增加到4周龄的水平，而A/J小鼠则没有。这与10天和2周时A/J组织中自噬标记物LC 3的免疫反应性相对应。在1周龄，10天和2周龄时检查基因表达的差异，因为在此期间发生了发散生长。1周龄时DBA/2 J颈动脉体中Msx 2的表达高于A/J颈动脉体。没有其他候选基因在年龄和菌株之间表现出一致的差异。1周龄的颈交感神经节中未见这种差异，提示这种差异是颈动脉体特异性的。目前的研究表明，关键的出生后时期，发展独特的形态，颈动脉体在这些小鼠。需要进一步的研究来进一步阐明Msx 2和其他未研究的基因的作用。
We have previously shown that the adult DBA/2J and A/J strains of mice differ in carotid body volume and morphology. The question has arisen whether these differences develop during the prenatal or postnatal period. Investigating morphological development of the carotid body and contributing genes in these mice can provide further understanding of the appropriate formation of the carotid body. We examined the carotid body of these mice from 1 day to 4 wk old for differences in volume, morphology, and gene expression of Gdnf family, Dlx2, Msx2, and Phox2b. The two strains showed divergent morphology starting at 1 wk old. The volume of the carotid body increased from 1 wk up to 2 wk old to the level of 4 wk old in the DBA/2J mice but not in the A/J mice. This corresponds with immunoreactivity of LC3, an autophagy marker, in A/J tissues at 10 days and 2 wk. The differences in gene expression were examined at 1 wk, 10 days, and 2 wk old, because divergent growth occurred during this period. The DBA/2J's carotid body at 1 wk old showed a greater expression of Msx2 than the A/J's carotid body. No other candidate genes showed consistent differences between the ages and strains. The difference was not seen in sympathetic cervical ganglia of 1 wk old, suggesting that the difference is carotid body specific. The current study indicates the critical postnatal period for developing distinctive morphology of the carotid body in these mice. Further studies are required to further elucidate a role of Msx2 and other uninvestigated genes.