Establishing a public health analytical service based on chemical methods for detecting and quantifying Pacific ciguatoxin in fish samples
Establishing a public health analytical service based on chemical methods for detecting and quantifying Pacific ciguatoxin in fish samples
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DOI：
10.1016/j.toxicon.2009.07.028
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发表时间：
2010-10-01
期刊：
影响因子：
2.8
通讯作者：
Shaw, Glen R.
中科院分区：
文献类型：
--
作者：
Stewart, Ian;Eaglesham, Geoffrey K.;Shaw, Glen R.
澳大利亚昆士兰州公共卫生分析实验室最近建立了一种用于检测和定量鱼肉中太平洋雪卡毒素的裁判分析方法。对 56 个鱼样本进行了分析，其中包括购买的 10 块鱼片作为阴性对照。在 27 个样品中发现了 P-CTX-1，在其中 26 个样品中发现了 P-CTX-2 和 P-CTX-3。 P-CTX-1浓度范围为0.04-11.4μg/kg鱼肉； 90 次重复分析的变异系数为 7.4%。利用快速甲醇萃取和净化的液相色谱/串联质谱 (HPLC-MS/MS) 方法可靠且重现性好，检测限为 0.03 mu g/kg 鱼肉。一些基质效应是明显的，鱼油含量可能是信号抑制因素。通过 DNA 序列分析对样品进行物种鉴定，发现了一些鱼类替代或无意的错误鉴定的证据，这可能对雪卡中毒的管理和预防产生影响。对疑似雪卡中毒病例的病例记录进行盲法检查表明，与雪卡相关的感觉异常的报告可以高度预测分析鱼中是否存在雪卡毒素，14 例预期病例中有 13 例食用了含有 P-CTX-1 的鱼（p < 0.001，渔民精确检验）。 Crown 版权所有 (C) 2009 由 Elsevier Ltd 出版。保留所有权利。
A referee analysis method for the detection and quantification of Pacific ciguatoxins in fish flesh has recently been established by the public health analytical laboratory for the State of Queensland, Australia. Fifty-six fish samples were analysed, which included 10 fillets purchased as negative controls. P-CTX-1 was identified in 27 samples, and P-CTX-2 and P-CTX-3 were found in 26 of those samples. The range of P-CTX-1 concentrations was 0.04-11.4 mu g/kg fish flesh; coefficient of variation from 90 replicate analyses was 7.4%. A liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) method utilising a rapid methanol extraction and clean-up is reliable and reproducible, with the detection limit at 0.03 mu g/kg fish flesh. Some matrix effects are evident, with fish oil content a likely signal suppression factor. Species identification of samples by DNA sequence analysis revealed some evidence of fish substitution or inadvertent misidentification, which may have implications for the management and prevention of ciguatera poisoning. Blinded inspection of case notes from suspect ciguatera poisoning cases showed that reporting of ciguatera-related paraesthesias was highly predictable for the presence of ciguatoxins in analysed fish, with 13 of 14 expected cases having consumed fish that contained P-CTX-1 (p < 0.001, Fishers Exact Test). Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.