Complex photonic response reveals three-dimensional self-organization of structural coloured bacterial colonies.
Complex photonic response reveals three-dimensional self-organization of structural coloured bacterial colonies.
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DOI：
10.1098/rsif.2020.0196
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发表时间：
2020-05-01
期刊：
影响因子：
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通讯作者：
Johansen, Villads E
中科院分区：
文献类型：
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作者：
Schertel, Lukas;van de Kerkhof, Gea T;Johansen, Villads E
在生物体中发现的鲜艳颜色通常是分层纳米结构散射的结果，其中排列有序和无序之间的相互作用决定了视觉外观。在黄杆菌IR1中，细胞在多晶三维晶格中的复杂排列被发现是集落组织的独特指纹。通过对体内细菌菌落角度分辨散射响应的分析分析与数值模拟相结合，我们表明，我们可以在10 nm以下的分辨率下评估细胞间距离和细胞直径，远远优于传统电子显微镜，受制备伪影的影响。从散射响应的显著特征中检索不同长度尺度上无序的作用，可以精确地理解细菌的结构组织。
Vivid colours found in living organisms are often the result of scattering from hierarchical nanostructures, where the interplay between order and disorder in their packing defines visual appearance. In the case of Flavobacterium IR1, the complex arrangement of the cells in polycrystalline three-dimensional lattices is found to be a distinctive fingerprint of colony organization. By combining analytical analysis of the angle-resolved scattering response of in vivo bacterial colonies with numerical modelling, we show that we can assess the inter-cell distance and cell diameter with a resolution below 10 nm, far better than what can be achieved with conventional electron microscopy, suffering from preparation artefacts. Retrieving the role of disorder at different length scales from the salient features in the scattering response enables a precise understanding of the structural organization of the bacteria.