Gamma radiation induced mutagenesis in Aspergillus niger to enhance its microbial fermentation activity for industrial enzyme production
Gamma radiation induced mutagenesis in Aspergillus niger to enhance its microbial fermentation activity for industrial enzyme production
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DOI：
10.1007/s11033-010-0239-3
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发表时间：
2011-02-01
影响因子：
2.8
通讯作者：
Rajoka, M. I.
中科院分区：
文献类型：
--
作者：
Awan, M. Siddique;Tabbasam, Nabila;Rajoka, M. I.
α-和β-半乳糖苷酶可应用于食品加工、健康和营养。尼日尔是这些酶的有效生产者之一，并使用γ射线诱导诱变进行基因型改良。该mu-衍生物产生两倍高的α-和β-半乳糖苷酶。为了检测两种菌株的遗传变异及其与表型性状的关系，对A.用28个十核苷酸合成引物扩增尼日尔。RAPD分析表明，亲本和突变体培养物之间的模式显着不同。突变体衍生物产生均一的扩增模式，而亲本菌株形成异质扩增模式。7个引物的扩增产物中有42.9%的多态性，表明这些引物决定了两个菌株之间的遗传变异。因此，RAPD被认为是一种有效的技术，以确定遗传变异的突变体和野生生物。分析了野生型和突变型菌株产生半乳糖苷酶的潜力。不同碳源对产酶量的影响比较表明，麦麸是产酶最有效的碳源，其次是米糠、大米精和乳糖（P < 0.01）。该突变株产酶能力显著提高，在食品、营养和保健方面具有潜在的应用价值，RAPD技术可有效地用于突变株与亲本菌株的鉴别。
alpha- and beta-Galactosidases find application in food processing, health and nutrition. Aspergillus niger is one of the potent producer of these enzymes and was genotypically improved using gamma-ray induced mutagenesis. The mutant-derivative produced two-fold higher alpha- and beta-galactosidases. For testing genetic variability and its relationship with phenotypic properties of the two organisms, DNA samples of the mutant and parental strains of A. niger were amplified with 28 deca-nucleotide synthetic primers. RAPD analysis showed significantly different pattern between parental and mutant cultures. The mutant derivative yielded homogeneous while parental strain formed heterogeneous amplification patterns. Seven primers identified 42.9% polymorphism in the amplification products, indicating that these primers determined some genetic variability between the two strains. Thus RAPD was found to be an efficient technique to determine genetic variability in the mutant and wild organisms. Both wild and mutant strains were analyzed for their potential to produce galactosidases. Comparison of different carbon sources on enzyme yield revealed that wheat bran is significant (P < 0.01) effective producer and economical source followed by rice bran, rice polishing and lactose. The mutant was significantly better enzyme producer and could be considered for its prospective application in food, nutrition and health and that RAPD can be effectively used to differentiate mutant strain from the parental strain based on the RAPD patterns.